ArticleBMC plant biology2013
Characterization of phosphorus-regulated miR399 and miR827 and their isomirs in barley under phosphorus-sufficient and phosphorus-deficient conditions.
Article in BMC plant biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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60 citing papers in PubMed, 130 citations in OpenAlex.
- Harnessing microRNA regulatory networks for engineering climate resilience and nutritional enhancement in millets.Stress biology · 2026Review
- Low phosphorus promotes caryophyllene biosynthesis by regulating AaTPS3 in Artemisia argyi.Plant cell reports · 2026Article
- Non-coding RNAs in plant stress responses: molecular insights and agricultural applications.Plant biotechnology journal · 2025Review
- Analysing the loss of embryogenic competence in long-term cell lines of Solanum betaceum Cav.: involvement of miR827, phosphate and sugar.BMC plant biology · 2025Article
- Decoding Long-Distance Communication Under Mineral Stress: Advances in Vascular Signalling and Molecular Tools for Plant Resilience.Plant, cell & environment · 2025Review
- The Emerging Applications of Artificial MicroRNA-Mediated Gene Silencing in Plant Biotechnology.Non-coding RNA · 2025Review
- Omics-Driven Strategies for Developing Saline-Smart Lentils: A Comprehensive Review.International journal of molecular sciences · 2024Review
- Identification of microRNAs involved in the Phosphate starvation response in Oil Palm (Elaeis guineensis Jacq.).Molecular biology reports · 2023Article
- INDETERMINATE1 autonomously regulates phosphate homeostasis upstream of the miR399-ZmPHO2 signaling module in maize.The Plant cell · 2023Article
- Genome-Wide Analysis of microRNA Expression Profile in Roots and Leaves of Three Wheat Cultivars under Water and Drought Conditions.Biomolecules · 2023Article
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- Comprehensive, integrative genomic analysis of microRNA expression profiles in different tissues of two wheat cultivars with different traits.Functional & integrative genomics · 2022Article
- Stu-miR827-TargetedInternational journal of molecular sciences · 2022Article
- Multi-omics reveals the key and specific miRNA-mRNA modules underlying salt tolerance in wild emmer wheat (Triticum dicoccoides L.).BMC genomics · 2022Article
- Transcriptome Level Analysis of Genes of Exogenous Ethylene Applied under Phosphorus Stress in Chinese Fir.Plants (Basel, Switzerland) · 2022Article
- Insight into the Roles of Proline-Rich Extensin-like Receptor Protein Kinases of Bread Wheat (Life (Basel, Switzerland) · 2022Article
- Identification of Sex Differentiation-Related microRNAs in Spinach Female and Male Flower.International journal of molecular sciences · 2022Article
- Identification and Functional Prediction of Poplar Root circRNAs Involved in Treatment With Different Forms of Nitrogen.Frontiers in plant science · 2022Article
- Integrated mRNA and microRNA expression analysis of root response to phosphate deficiency inFrontiers in plant science · 2022Article
- A novel plant-fungal association reveals fundamental sRNA and gene expression reprogramming at the onset of symbiosis.BMC biology · 2021Article
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Authors and funding
4 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundmiR399 and miR827 are both involved in conserved phosphorus (P) deficiency signalling pathways. miR399 targets the PHO2 gene encoding E2 enzyme that negatively regulates phosphate uptake and root-to-shoot allocation, while miR827 targets SPX-domain-containing genes that negatively regulate other P-responsive genes. However, the response of miR399 and miR827 to P conditions in barley has not been investigated.
resultsIn this study, we investigated the expression profiles of miR399 and miR827 in barley (Hordeum vulagre L.) under P-deficient and P-sufficient conditions. We identified 10 members of the miR399 family and one miR827 gene in barley, all of which were significantly up-regulated under deficient P. In addition, we found many isomirs of the miR399 family and miR827, most of which were also significantly up-regulated under deficient P. Several isomirs of miR399 members were found to be able to cleave their predicted targets in vivo. Surprisingly, a few small RNAs (sRNAs) derived from the single-stranded loops of the hairpin structures of MIR399b and MIR399e-1 were also found to be able to cleave their predicted targets in vivo. Many antisense sRNAs of miR399 and a few for miR827 were also detected, but they did not seem to be regulated by P. Intriguingly, the lowest expressed member, hvu-miR399k, had four-fold more antisense sRNAs than sense sRNAs, and furthermore under P sufficiency, the antisense sRNAs are more frequent than the sense sRNAs. We identified a potential regulatory network among miR399, its target HvPHO2 and target mimics HvIPS1 and HvIPS2 in barley under P-deficient and P-sufficient conditions.
conclusionsOur data provide an important insight into the mechanistic regulation and function of miR399, miR827 and their isomirs in barley under different P conditions.
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