ArticleMicrobial biotechnology2014
Engineering of a green-light inducible gene expression system in Synechocystis sp. PCC6803.
Article in Microbial biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 78 citations in OpenAlex.
- Harnessing engineered cyanobacteria for next-generation therapeutics.Archives of microbiology · 2026Review
- Optogenetic control of gene expression in the cyanobacteriumFrontiers in bioengineering and biotechnology · 2024Article
- Responding to light signals: a comprehensive update on photomorphogenesis in cyanobacteria.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Review
- A Blue Light-Responsive Strong Synthetic Promoter Based on Rational Design inInternational journal of molecular sciences · 2023Article
- Highlighter: An optogenetic system for high-resolution gene expression control in plants.PLoS biology · 2023Article
- Identification of a Far-Red Light-Inducible Promoter that Exhibits Light Intensity Dependency and Reversibility in a Cyanobacterium.ACS synthetic biology · 2023Article
- Current Metabolic Engineering Strategies for Photosynthetic Bioproduction in Cyanobacteria.Microorganisms · 2023Review
- The Molecular Toolset and Techniques Required to Build Cyanobacterial Cell Factories.Advances in biochemical engineering/biotechnology · 2023Article
- Optogenetics in bacteria - applications and opportunities.FEMS microbiology reviews · 2022Review
- Development of Optogenetic Dual-Switch System for Rewiring Metabolic Flux for Polyhydroxybutyrate Production.Molecules (Basel, Switzerland) · 2022Article
- Light-induced production of isobutanol and 3-methyl-1-butanol by metabolically engineered cyanobacteria.Microbial cell factories · 2022Article
- Light-regulated gene expression in Bacteria: Fundamentals, advances, and perspectives.Frontiers in bioengineering and biotechnology · 2022Review
- The Red Edge: Bilin-Binding Photoreceptors as Optogenetic Tools and Fluorescence Reporters.Chemical reviews · 2021Review
- Artificial complementary chromatic acclimation gene expression system in Escherichia coli.Microbial cell factories · 2021Article
- Heterologous production of cyanobacterial compounds.Journal of industrial microbiology & biotechnology · 2021Review
- A Green Light-Regulated T7 RNA Polymerase Gene Expression System for Cyanobacteria.Marine biotechnology (New York, N.Y.) · 2021Article
- Regulatory systems for gene expression control in cyanobacteria.Applied microbiology and biotechnology · 2020Review
- Progress and challenges in engineering cyanobacteria as chassis for light-driven biotechnology.Microbial biotechnology · 2020Article
- Emerging Species and Genome Editing Tools: Future Prospects in Cyanobacterial Synthetic Biology.Microorganisms · 2019Review
- The Synthetic Biology Toolkit for Photosynthetic Microorganisms.Plant physiology · 2019Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to construct a green-light-regulated gene expression system for cyanobacteria, we characterized a green-light sensing system derived from Synechocystis sp. PCC6803, consisting of the green-light sensing histidine kinase CcaS, the cognate response regulator CcaR, and the promoter of cpcG2 (PcpcG 2 ). CcaS and CcaR act as a genetic controller and activate gene expression from PcpcG 2 with green-light illumination. The green-light induction level of the native PcpcG 2 was investigated using GFPuv as a reporter gene inserted in a broad-host-range vector. A clear induction of protein expression from native PcpcG 2 under green-light illumination was observed; however, the expression level was very low compared with Ptrc , which was reported to act as a constitutive promoter in cyanobacteria. Therefore, a Shine-Dalgarno-like sequence derived from the cpcB gene was inserted in the 5' untranslated region of the cpcG2 gene, and the expression level of CcaR was increased. Thus, constructed engineered green-light sensing system resulted in about 40-fold higher protein expression than with the wild-type promoter with a high ON/OFF ratio under green-light illumination. The engineered green-light gene expression system would be a useful genetic tool for controlling gene expression in the emergent cyanobacterial bioprocesses.
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