ArticleParasites & vectors2013
The ovarian transcriptome of the cattle tick, Rhipicephalus (Boophilus) microplus, feeding upon a bovine host infected with Babesia bovis.
Article in Parasites & vectors, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 34 citations in OpenAlex.
- Infection with Babesia bovis alters metabolic rates of Rhipicephalus microplus ticks across life stages.Parasites & vectors · 2025Article
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- Transcriptomic analysis of Rhipicephalus microplus hemocytes from female ticks infected with Babesia bovis or Babesia bigemina.Parasites & vectors · 2025Article
- A multi-omics approach for understanding blood digestion dynamics in Ixodes scapularis and identification of anti-tick vaccine targets.Ticks and tick-borne diseases · 2024Article
- More than Three Decades of Bm86: What We Know and Where to Go.Pathogens (Basel, Switzerland) · 2023Review
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- Experimental Infection of Ticks: An Essential Tool for the Analysis ofPathogens (Basel, Switzerland) · 2021Review
- A physiologic overview of the organ-specific transcriptome of the cattle tick Rhipicephalus microplus.Scientific reports · 2020Article
- Cathepsin L-a novel cysteine protease from Haemaphysalis flava Neumann, 1897.Parasitology research · 2019Article
- The multiple roles of peroxiredoxins in tick blood feeding.Experimental & applied acarology · 2018Review
- A proteomic insight into vitellogenesis during tick ovary maturation.Scientific reports · 2018Article
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- Tick-Pathogen Interactions and Vector Competence: Identification of Molecular Drivers for Tick-Borne Diseases.Frontiers in cellular and infection microbiology · 2017Review
- Sialomes and Mialomes: A Systems-Biology View of Tick Tissues and Tick-Host Interactions.Trends in parasitology · 2016Review
- Saliva from nymph and adult females of Haemaphysalis longicornis: a proteomic study.Parasites & vectors · 2015Article
- Transmission-Blocking Vaccines: Focus on Anti-Vector Vaccines against Tick-Borne Diseases.Archivum immunologiae et therapiae experimentalis · 2015Review
- Deep Sequencing Analysis of the Ixodes ricinus Haemocytome.PLoS neglected tropical diseases · 2015Article
- Rhipicephalus microplus serine protease inhibitor family: annotation, expression and functional characterisation assessment.Parasites & vectors · 2015Article
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCattle babesiosis is a tick-borne disease of cattle with the most severe form of the disease caused by the apicomplexan, Babesia bovis. Babesiosis is transmitted to cattle through the bite of infected cattle ticks of the genus Rhipicephalus. The most prevalent species is Rhipicephalus (Boophilus) microplus, which is distributed throughout the tropical and subtropical countries of the world. The transmission of B. bovis is transovarian and a previous study of the R. microplus ovarian proteome identified several R. microplus proteins that were differentially expressed in response to infection. Through various approaches, we studied the reaction of the R. microplus ovarian transcriptome in response to infection by B. bovis.
methodsA group of ticks were allowed to feed on a B. bovis-infected splenectomized calf while a second group fed on an uninfected splenectomized control calf. RNA was purified from dissected adult female ovaries of both infected and uninfected ticks and a subtracted B. bovis-infected cDNA library was synthesized, subtracting with the uninfected ovarian RNA. Four thousand ESTs were sequenced from the ovary subtracted library and annotated.
resultsThe subtracted library dataset assembled into 727 unique contigs and 2,161 singletons for a total of 2,888 unigenes, Microarray experiments designed to detect B. bovis-induced gene expression changes indicated at least 15 transcripts were expressed at a higher level in ovaries from ticks feeding upon the B. bovis-infected calf as compared with ovaries from ticks feeding on an uninfected calf. We did not detect any transcripts from these microarray experiments that were expressed at a lower level in the infected ovaries compared with the uninfected ovaries. Using the technique called serial analysis of gene expression, 41 ovarian transcripts from infected ticks were differentially expressed when compared with transcripts of controls.
conclusionCollectively, our experimental approaches provide the first comprehensive profile of the R. microplus ovarian transcriptome responding to infection by B. bovis. This dataset should prove useful in molecular studies of host-pathogen interactions between this tick and its apicomplexan parasite.
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