ArticleBMC genomics2013
Sequencing the transcriptome of milk production: milk trumps mammary tissue.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01817127 (Functional Deconstruction of Human Milk Oligosaccharides and Lipids), which is not on this map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Functional Deconstruction of Human Milk Oligosaccharides and Lipids
Who cites it
38 citing papers in PubMed, 60 citations in OpenAlex.
- Preserving RNA quality when freezing of human milk samples must occur before extraction: Validation of methods utilized in a multi-center cohort study.Journal of mammary gland biology and neoplasia · 2026Article
- Comparison of 4 different RNA sources from lactating dairy cows to assess the mammary transcript abundance.JDS communications · 2025Article
- Transcriptomic profiling of milk fat globules in cows with different β-casein genotypes.Scientific reports · 2025Article
- Characterization of mammary glands and milk fat globule transcripts in lactating buffalo and goats.Food chemistry. Molecular sciences · 2025Article
- Preservation of milk in liquid nitrogen during sample collection does not affect the RNA quality for RNA-seq analysis.BMC genomics · 2025Article
- Transcriptome analysis of mammary epithelial cell between Sewa sheep and East FriEsian sheep from different localities.BMC genomics · 2024Article
- Human milk variation is shaped by maternal genetics and impacts the infant gut microbiome.Cell genomics · 2024Article
- Transcriptomic analysis reveals molecular insights into lactation dynamics in Jakhrana goat mammary gland.BMC genomics · 2024Article
- Human cytomegalovirus in breast milk is associated with milk composition and the infant gut microbiome and growth.Nature communications · 2024Article
- Current status and challenges for cell-cultured milk technology: a systematic review.Journal of animal science and biotechnology · 2024Review
- Importance of human milk for infants in the clinical setting: Updates and mechanistic links.Nutrition in clinical practice : official publication of the American Society for Parenteral and Enteral Nutrition · 2023Review
- Human Cytomegalovirus in breast milk is associated with milk composition, the infant gut microbiome, and infant growth.bioRxiv : the preprint server for biology · 2023Article
- Genomic and Phenotypic Udder Evaluation for Dairy Cattle Selection: A Review.Animals : an open access journal from MDPI · 2023Review
- Lactoferrin, Osteopontin and Lactoferrin-Osteopontin Complex: A Critical Look on Their Role in Perinatal Period and Cardiometabolic Disorders.Nutrients · 2023Review
- Fur removal promotes an earlier expression of involution-related genes in mammary gland of lactating mice.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2023Article
- Excavation and characterization of key circRNAs for milk fat percentage in Holstein cattle.Journal of animal science · 2023Article
- The Mothers, Infants, and Lactation Quality (MILQ) Study: A Multi-Center Collaboration.Current developments in nutrition · 2021Article
- Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities.BMC genomic data · 2021Article
- A Comparative Review of the Cell Biology, Biochemistry, and Genetics of Lactose Synthesis.Journal of mammary gland biology and neoplasia · 2021Review
- Best Practices for Human Milk Collection for COVID-19 Research.Breastfeeding medicine : the official journal of the Academy of Breastfeeding Medicine · 2021Review
Corrections and comments
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
Abstract
backgroundStudies of normal human mammary gland development and function have mostly relied on cell culture, limited surgical specimens, and rodent models. Although RNA extracted from human milk has been used to assay the mammary transcriptome non-invasively, this assay has not been adequately validated in primates. Thus, the objectives of the current study were to assess the suitability of lactating rhesus macaques as a model for lactating humans and to determine whether RNA extracted from milk fractions is representative of RNA extracted from mammary tissue for the purpose of studying the transcriptome of milk-producing cells.
resultsWe confirmed that macaque milk contains cytoplasmic crescents and that ample high-quality RNA can be obtained for sequencing. Using RNA sequencing, RNA extracted from macaque milk fat and milk cell fractions more accurately represented RNA from mammary epithelial cells (cells that produce milk) than did RNA from whole mammary tissue. Mammary epithelium-specific transcripts were more abundant in macaque milk fat, whereas adipose or stroma-specific transcripts were more abundant in mammary tissue. Functional analyses confirmed the validity of milk as a source of RNA from milk-producing mammary epithelial cells.
conclusionsRNA extracted from the milk fat during lactation accurately portrayed the RNA profile of milk-producing mammary epithelial cells in a non-human primate. However, this sample type clearly requires protocols that minimize RNA degradation. Overall, we validated the use of RNA extracted from human and macaque milk and provided evidence to support the use of lactating macaques as a model for human lactation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.