ArticleParasites & vectors2013
Multiplication of the waterborne pathogen Cryptosporidium parvum in an aquatic biofilm system.
Article in Parasites & vectors, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- The functional landscape of the appendix microbiome under conditions of health and disease.Gut pathogens · 2025Review
- Unraveling the interplay between unicellular parasites and bacterial biofilms: Implications for disease persistence and antibiotic resistance.Virulence · 2024Review
- Legionella colonization and 3D spatial location within a Pseudomonas biofilm.Scientific reports · 2024Article
- Ecology ofBiofilm · 2024Review
- Intelligent Biological Networks: Improving Anti-Microbial Resistance Resilience through Nutritional Interventions to Understand Protozoal Gut Infections.Microorganisms · 2023Review
- Interactions between free-living amoebae and Cryptosporidium parvum: an experimental study.Parasite (Paris, France) · 2023Article
- Cryptosporidiosis Modulates the Gut Microbiome and Metabolism in a Murine Infection Model.Metabolites · 2021Article
- Detection of Giardia and Cryptosporidium in environmental matrices with immunomagnetic separation: two or three acid dissociations.Parasitology research · 2021Article
- Review
- Biofilm Sampling for Detection ofApplied and environmental microbiology · 2020Article
- Calcium-Mediated Biophysical Binding of Cryptosporidium parvum Oocysts to Surfaces Is Sensitive to Oocyst Age.Applied and environmental microbiology · 2019Article
- Past and future trends of Cryptosporidium in vitro research.Experimental parasitology · 2019Review
- Performance comparison of three methods for detection of Giardia spp. cysts and Cryptosporidium spp. oocysts in drinking-water treatment sludge.Environmental monitoring and assessment · 2018Article
- Assessment and Molecular Characterization of Human Intestinal Parasites in Bivalves from Orchard Beach, NY, USA.International journal of environmental research and public health · 2016Article
- Glycoproteins and Gal-GalNAc cause Cryptosporidium to switch from an invasive sporozoite to a replicative trophozoite.International journal for parasitology · 2016Article
- The pulsed light inactivation of veterinary relevant microbial biofilms and the use of a RTPCR assay to detect parasite species within biofilm structures.Open veterinary journal · 2016Article
- Quantitative analysis of Cryptosporidium growth in in vitro culture--the impact of parasite density on the success of infection.Parasitology research · 2016Article
- Extracellular excystation and development of Cryptosporidium: tracing the fate of oocysts within Pseudomonas aquatic biofilm systems.BMC microbiology · 2014Article
- The expression dynamics of IL-17 and Th17 response relative cytokines in the trachea and spleen of chickens after infection with Cryptosporidium baileyi.Parasites & vectors · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn natural aquatic environments biofilms are known to act as environmental reservoirs for Cryptosporidium parvum oocysts. However, the fate of these oocysts within biofilms has yet to be determined.
methodsThis study aimed to identify if biofilms have the ability to support the multiplication of Cryptosporidium by measuring the change in parasite number over time using quantitative polymerase chain reaction (qPCR) and detecting the possible extracellular developmental stages using a combination of confocal microscopy and immunolabelling techniques. Pseudomonas aeruginosa biofilm flow cell systems were established and C. parvum oocysts were constantly supplied over a six day period.
resultsA significant (P<0.001) increase in Cryptosporidium was detected as the biofilm matured, with the total number of C. parvum multiplying 2-3 fold during this period. With this, various Cryptosporidium developmental stages (sporozoites, trophozoites, type I and II meronts) were identified from the biofilm.
conclusionThis is the first study demonstrating that biofilms not only serve as an environmental reservoir for oocysts, but are also capable of supporting the multiplication of Cryptosporidium over time in an aquatic environment.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.