ArticleBMC complementary and alternative medicine2013
In vitro neuroprotective potential of four medicinal plants against rotenone-induced toxicity in SH-SY5Y neuroblastoma cells.
Article in BMC complementary and alternative medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 42 citations in OpenAlex.
- The Phytochemistry and Pharmacology ofMolecules (Basel, Switzerland) · 2022Review
- Phyto-Carbazole Alkaloids from the Rutaceae Family as Potential Protective Agents against Neurodegenerative Diseases.Antioxidants (Basel, Switzerland) · 2022Review
- Review
- Neuroprotective Activities ofMolecules (Basel, Switzerland) · 2020Article
- Biophysical and biomechanical properties of neural progenitor cells as indicators of developmental neurotoxicity.Archives of toxicology · 2019Article
- Neuroprotective effect of Mayan medicinal plant extracts against glutamate-induced toxicity.Journal of natural medicines · 2019Article
- Vildagliptine protects SH-SY5Y human neuron-like cells from Aβ 1-42 induced toxicity, in vitro.Cytotechnology · 2019Article
- Review of Ethnomedicinal, Phytochemical and Pharmacological Properties ofMolecules (Basel, Switzerland) · 2019Review
- Chemical composition, antioxidant potential, macromolecule damage and neuroprotective activity ofJournal of traditional and complementary medicine · 2018Article
- Neuroprotective Effects of Protein Tyrosine Phosphatase 1B Inhibition against ER Stress-Induced Toxicity.Molecules and cells · 2017Article
- Endoplasmic Reticulum Stress Plays a Key Role in Rotenone-Induced Apoptotic Death of Neurons.Molecular neurobiology · 2016Article
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3 authors at 1 institution in 1 country.
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Abstract
backgroundLannea schweinfurthii, Zanthoxylum capense, Scadoxus puniceus and Crinum bulbispermum are used traditionally to treat neurological disorders. The aim of this study was to evaluate the cytoprotective potential of the four plants, after induction of toxicity using rotenone, in SH-SY5Y neuroblastoma cells.
methodsCytotoxicity of the plant extracts and rotenone was assessed using the sulforhodamine B (SRB) assay. Fluorometry was used to measure intracellular redox state (reactive oxygen species (ROS) and intracellular glutathione content), mitochondrial membrane potential (MMP) and caspase-3 activity, as a marker of apoptotic cell death.
resultsOf the tested plants, the methanol extract of Z. capense was the least cytotoxic; LC50 121.3 ± 6.97 μg/ml, while S. puniceus methanol extract was the most cytotoxic; LC50 20.75 ± 1.47 μg/ml. Rotenone reduced intracellular ROS levels after 24 h exposure. Pre-treating cells with S. puniceus and C. bulbispermum extracts reversed the effects of rotenone on intracellular ROS levels. Rotenone exposure also decreased intracellular glutathione levels, which was counteracted by pre-treatment with any one of the extracts. MMP was reduced by rotenone, which was neutralized by pre-treatment with C. bulbispermum ethyl acetate extract. All extracts inhibited rotenone-induced activation of caspase-3.
conclusionThe studied plants demonstrated anti-apoptotic activity and restored intracellular glutathione content following rotenone treatment, suggesting that they may possess neuroprotective properties.
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