ArticleMolecular microbiology2014
Enzymes involved in plastid-targeted phosphatidic acid synthesis are essential for Plasmodium yoelii liver-stage development.
Article in Molecular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 68 citations in OpenAlex.
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- Integrated lipidomic and transcriptomic profiling of the host response in human malaria.Genome biology · 2026Article
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- Liver stage P. falciparum antigens highly targeted by CD4+ T cells in malaria-exposed Ugandan children.PLoS pathogens · 2025Article
- Pivotal roles of Plasmodium falciparum lysophospholipid acyltransferase 1 in cell cycle progression and cytostome internalization.Communications biology · 2025Article
- A kalihinol analog disrupts apicoplast function and vesicular trafficking inScience (New York, N.Y.) · 2024Article
- Complex Endosymbiosis II: The Nonphotosynthetic Plastid of Apicomplexa Parasites (The Apicoplast) and Its Integrated Metabolism.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Creation and preclinical evaluation of genetically attenuated malaria parasites arresting growth late in the liver.NPJ vaccines · 2022Article
- Identification of GmGPATs and their effect on glycerolipid biosynthesis through seed-specific expression in soybean.Molecular biology reports · 2022Article
- The flexibility of Apicomplexa parasites in lipid metabolism.PLoS pathogens · 2022Review
- Characterization of functions in parasite growth and virulence of four Toxoplasma gondii genes involved in lipid synthesis by CRISPR-Cas9 system.Parasitology research · 2021Article
- Definition of constitutive and stage-enriched promoters in the rodent malaria parasite, Plasmodium yoelii.Malaria journal · 2020Article
- Transcriptomics and proteomics reveal two waves of translational repression during the maturation of malaria parasite sporozoites.Nature communications · 2019Article
- Opportunities for Host-targeted Therapies for Malaria.Trends in parasitology · 2018Review
- Article
- Plasmodium parasite exploits host aquaporin-3 during liver stage malaria infection.PLoS pathogens · 2018Article
- A Plasmodium Parasite with Complete Late Liver Stage Arrest Protects against Preerythrocytic and Erythrocytic Stage Infection in Mice.Infection and immunity · 2018Article
- Puf3 participates in ribosomal biogenesis in malaria parasites.Journal of cell science · 2018Article
- Lipid analysis ofCell discovery · 2018Article
- A Novel Complementation Assay for Quick and Specific Screen of Genes Encoding Glycerol-3-Phosphate Acyltransferases.Frontiers in plant science · 2018Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Malaria parasites scavenge nutrients from their host but also harbour enzymatic pathways for de novo macromolecule synthesis. One such pathway is apicoplast-targeted type II fatty acid synthesis, which is essential for late liver-stage development in rodent malaria. It is likely that fatty acids synthesized in the apicoplast are ultimately incorporated into membrane phospholipids necessary for exoerythrocytic merozoite formation. We hypothesized that these synthesized fatty acids are being utilized for apicoplast-targeted phosphatidic acid synthesis, the phospholipid precursor. Phosphatidic acid is typically synthesized in a three-step reaction utilizing three enzymes: glycerol 3-phosphate dehydrogenase, glycerol 3-phosphate acyltransferase and lysophosphatidic acid acyltransferase. The Plasmodium genome is predicted to harbour genes for both apicoplast- and cytosol/endoplasmic reticulum-targeted phosphatidic acid synthesis. Our research shows that apicoplast-targeted Plasmodium yoelii glycerol 3-phosphate dehydrogenase and glycerol 3-phosphate acyltransferase are expressed only during liver-stage development and deletion of the encoding genes resulted in late liver-stage growth arrest and lack of merozoite differentiation. However, the predicted apicoplast-targeted lysophosphatidic acid acyltransferase gene was refractory to deletion and was expressed solely in the endoplasmic reticulum throughout the parasite life cycle. Our results suggest that P. yoelii has an incomplete apicoplast-targeted phosphatidic acid synthesis pathway that is essential for liver-stage maturation.
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