ArticleTraffic (Copenhagen, Denmark)2014
Trs20 is required for TRAPP III complex assembly at the PAS and its function in autophagy.
Article in Traffic (Copenhagen, Denmark), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- A genome-wide CRISPR knockout screen identified host genes essential for Brucella invasion and intracellular survival.Emerging microbes & infections · 2026Article
- Establishment of the phagophore-ERES membrane contact site initiates phagophore elongation.Nature structural & molecular biology · 2025Article
- The autophagy-specific exocyst subcomplex contributes to phagophore assembly site integrity by promoting phagophore expansion.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Establishment of a yeast essential protein conditional-degradation library and screening for autophagy-regulating genes.Autophagy · 2025Article
- TRAPPC2l Participates in Male Germ Cell Development by Regulating Cell Division.Cell proliferation · 2025Article
- TRAPPopathies: Severe Multisystem Disorders Caused by Variants in Genes of the Transport Protein Particle (TRAPP) Complexes.International journal of molecular sciences · 2024Review
- Rab1b facilitates lipid droplet growth by ER-to-lipid droplet targeting of DGAT2.Science advances · 2024Article
- The TRAPP complexes: oligomeric exchange factors that activate the small GTPases Rab1 and Rab11.FEBS letters · 2023Review
- The TRAPP complexes: discriminating GTPases in context.FEBS letters · 2023Review
- Cryo-EM structure of metazoan TRAPPIII, the multi-subunit complex that activates the GTPase Rab1.The EMBO journal · 2021Article
- Structural basis of TRAPPIII-mediated Rab1 activation.The EMBO journal · 2021Article
- Deficiencies in vesicular transport mediated by TRAPPC4 are associated with severe syndromic intellectual disability.Brain : a journal of neurology · 2020Article
- Characterization of Aspergillus nidulans TRAPPs uncovers unprecedented similarities between fungi and metazoans and reveals the modular assembly of TRAPPII.PLoS genetics · 2019Article
- Ypt/Rab GTPases and their TRAPP GEFs at the Golgi.FEBS letters · 2019Review
- Exocyst Subcomplex Functions in Autophagosome Biogenesis by Regulating Atg9 Trafficking.Journal of molecular biology · 2019Article
- Small GTPase proteins in macroautophagy.Small GTPases · 2018Review
- The two TRAPP complexes of metazoans have distinct roles and act on different Rab GTPases.The Journal of cell biology · 2018Article
- TRAPPC13 modulates autophagy and the response to Golgi stress.Journal of cell science · 2017Article
- Article
- TRAPP Complexes in Secretion and Autophagy.Frontiers in cell and developmental biology · 2016Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The modular TRAPP complex acts as a guanine-nucleotide exchange factor (GEF) for Ypt/Rab GTPases. Whereas TRAPP I and TRAPP II regulate the exocytic pathway, TRAPP III functions in autophagy. The TRAPP subunit Trs20 is not required for assembly of core TRAPP or its Ypt1 GEF activity. Interestingly, mutations in the human functional ortholog of Trs20, Sedlin, cause spondyloepiphyseal dysplasia tarda (SEDT), a cartilage-specific disorder. We have shown that Trs20 is required for TRAPP II assembly and identified a SEDT-linked mutation, Trs20-D46Y, which causes a defect in this process. Here we show that Trs20 is also required for assembly of TRAPP III at the pre-autophagosomal structure (PAS). First, recombinant Trs85, a TRAPP III-specific subunit, associates with TRAPP only in the presence of Trs20, but not Trs20-D46Y mutant protein. Second, a TRAPP complex with Ypt1 GEF activity co-precipitates with Trs85 from wild type, but not trs20ts mutant, cell lysates. Third, live-cell colocalization analysis indicates that Trs85 recruits core TRAPP to the PAS via the linker protein Trs20. Finally, trs20ts mutant cells are defective in selective and non-selective autophagy. Together, our results show that Trs20 plays a role as an adaptor in the assembly of TRAPP II and TRAPP III complexes, and the SEDT-linked mutation causes a defect in both processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.