Evidence map›Paper›PMID 24328564›Full record

ArticleNucleosides, nucleotides & nucleic acids2013

5'-deoxy-5'-hydrazinylguanosine as an initiator of T7 Rna polymerase-catalyzed transcriptions for the preparation of labeling-ready RNAs.

Mark Skipsey, Gordon Hack, Thomas A Hooper, Mark C Shankey, Louis P Conway, Martin Schröder, David R W Hodgson

Abstract read
In one paragraph

Article in Nucleosides, nucleotides & nucleic acids, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. A T7 RNA Polymerase Mutant Enhances the Yield of 5'-Thienoguanosine-Initiated RNAs.Chembiochem : a European journal of chemical biology · 2018
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Mark Skipseya Department of Chemistry and Biophysical Sciences Institute , Durham University , Durham , United Kingdom.
Gordon Hack
Thomas A Hooper
Mark C Shankey
Louis P Conway
Martin Schröder
David R W Hodgson

Funding

Biotechnology and Biological Sciences Research Council BB/C/514582/1Biotechnology and Biological Sciences Research Council BB/C514582/1Biotechnology and Biological Sciences Research Council BB/D01588X/1Biotechnology and Biological Sciences Research Council BB/F003757/1
6 · The paper itself

Abstract

5'-deoxy-5'-hydrazinylguanosine was incorporated into the 5'-termini of RNA transcripts using T7 RNA polymerase. Transcriptions provided 5'-hydrazinyl-RNA that was readily labeled and purified. The use of fluorophore-labeled material was validated in an endoribonuclease activity assay.

Indexed as

Bacteriophage T7Base SequenceDNA-Directed RNA PolymerasesFluorescein-5-isothiocyanateFluorescent DyesGuanosineHydrazinesMolecular Sequence DataRNATranscription, GeneticViral Proteinsbacteriophage T7 RNA polymeraseDNA-Directed RNA PolymerasesFluorescein-5-isothiocyanateFluorescent DyesGuanosineHydrazinesRNAViral Proteins

Identifiers

PMID24328564
PMCPMC3924349

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.