ArticleLangmuir : the ACS journal of surfaces and colloids2013
DNA-modified electrodes fabricated using copper-free click chemistry for enhanced protein detection.
Article in Langmuir : the ACS journal of surfaces and colloids, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 44 citations in OpenAlex.
- Complementary cost-effective electrochemical platforms for point-of-use biosensing.Advanced sensor research · 2025Article
- Stability of N-Heterocyclic Carbene Monolayers under Continuous Voltammetric Interrogation.ACS applied materials & interfaces · 2023Article
- On-Surface Azide-Alkyne Cycloaddition Reaction: Does It Click with Ruthenium Catalysts?Langmuir : the ACS journal of surfaces and colloids · 2022Article
- Surface Requirements for Optimal Biosensing with Disposable Gold Electrodes.ACS measurement science au · 2022Article
- DNA Electrochemistry: Charge-Transport Pathways through DNA Films on Gold.Journal of the American Chemical Society · 2021Article
- Sulfamide chemistry applied to the functionalization of self-assembled monolayers on gold surfaces.Beilstein journal of organic chemistry · 2017Article
- Electrical Probes of DNA-Binding Proteins.Methods in enzymology · 2017Article
- DNA Charge Transport: from Chemical Principles to the Cell.Cell chemical biology · 2016Review
- Nucleotidyl transferase assisted DNA labeling with different click chemistries.Nucleic acids research · 2015Article
- A Multiplexed, Two-Electrode Platform for Biosensing Based on DNA-Mediated Charge Transport.Langmuir : the ACS journal of surfaces and colloids · 2015Article
- Electrochemistry of nonconjugated proteins and glycoproteins. Toward sensors for biomedicine and glycomics.Chemical reviews · 2015Review
- Electrocatalysis in DNA Sensors.Polyhedron · 2014Article
- Label-free electrochemical detection of human methyltransferase from tumors.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
A method of DNA monolayer formation has been developed using copper-free click chemistry that yields enhanced surface homogeneity and enables variation in the amount of DNA assembled; extremely low-density DNA monolayers, with as little as 5% of the monolayer being DNA, have been formed. These DNA-modified electrodes (DMEs) were characterized visually, with AFM, and electrochemically, and were found to facilitate DNA-mediated reduction of a distally bound redox probe. These low-density monolayers were found to be more homogeneous than traditional thiol-modified DNA monolayers, with greater helix accessibility through an increased surface area-to-volume ratio. Protein binding efficiency of the transcriptional activator TATA-binding protein (TBP) was also investigated on these surfaces and compared to that on DNA monolayers formed with standard thiol-modified DNA. Our low-density monolayers were found to be extremely sensitive to TBP binding, with a signal decrease in excess of 75% for 150 nM protein. This protein was detectable at 4 nM, on the order of its dissociation constant, with our low-density monolayers. The improved DNA helix accessibility and sensitivity of our low-density DNA monolayers to TBP binding reflects the general utility of this method of DNA monolayer formation for DNA-based electrochemical sensor development.
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Registered trials
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