ArticleThe Korean journal of parasitology2013
Detection of acute toxoplasmosis in pigs using loop-mediated isothermal amplification and quantitative PCR.
Article in The Korean journal of parasitology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Recent advances in isothermal amplification techniques for detection of animal diseases.Frontiers in veterinary science · 2026Review
- Evolution ofJournal of parasitology research · 2026Review
- First report of molecular detection and phylogenetic analysis of Toxoplasma Gondii in soil, water and vegetables from Chandigarh city, India.Scientific reports · 2025Article
- Clinical validation of visual LAMP and qLAMP assays for the rapid detection ofFrontiers in cellular and infection microbiology · 2022Article
- RAA-Cas12a-Tg: A Nucleic Acid Detection System forMicroorganisms · 2021Article
- Advances in serological, imaging techniques and molecular diagnosis of Toxoplasma gondii infection.Infection · 2018Review
- Molecular detection and phylogenetic analyses ofVeterinary medicine and science · 2017Article
- Molecular detection ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2016Article
- Diagnosis of toxoplasmosis and typing of Toxoplasma gondii.Parasites & vectors · 2015Review
- Loop-mediated isothermal amplification for rapid and semiquantitative detection of Loa loa infection.Journal of clinical microbiology · 2014Article
- DNA Amplification Techniques for the Detection ofIranian journal of parasitologyReview
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Authors and funding
5 authors.
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Abstract
A loop-mediated isothermal amplification (LAMP) assay allows rapid diagnosis of Toxoplasma gondii infection. In the present study, the LAMP assay was evaluated using blood from both naturally and experimentally infected pigs. The sensitivity of the LAMP assay was compared with that of Q-PCR. Both assays detected T. gondii in the blood of experimentally infected pigs, with 100% agreement. In infected blood samples, the parasite was detected as early as 2 days post-infection and reached a peak in 3-5 days. In 216 field serum samples, the detection rates of LAMP and Q-PCR assays were 6.9% and 7.8%, respectively. This result indicates that the sensitivity of the LAMP assay was slightly lower than that of the Q-PCR assay. However, the LAMP may be an attractive diagnostic method in conditions where sophisticated and expensive equipment is unavailable. This assay could be a powerful supplement to current diagnostic methods.
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