ArticleThe Journal of biological chemistry2014
The D-ring, not the A-ring, rotates in Synechococcus OS-B' phytochrome.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 52 citations in OpenAlex.
- Preparation of Phycocyanobilin (PCB) with and withoutMethods in molecular biology (Clifton, N.J.) · 2026Article
- Circular dichroism spectroscopy reveals multiple phytochrome photoproducts in equilibrium.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2025Article
- Integrated Study of Fluorescence Enhancement in the Y176H Variant of Cyanobacterial Phytochrome Cph1.Biochemistry · 2025Article
- Crystal structure of the photosensory module from a PAS-less cyanobacterial phytochrome as Pr shows a mix of dark-adapted and photoactivated features.The Journal of biological chemistry · 2024Article
- Evidence for an early green/red photocycle that precedes the diversification of GAF domain photoreceptor cyanobacteriochromes.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2023Article
- Modulation of biliverdin dynamics and spectral properties by Sandercyanin.RSC advances · 2022Article
- Improved fluorescent phytochromes for in situ imaging.Scientific reports · 2022Article
- Protein-chromophore interactions controlling photoisomerization in red/green cyanobacteriochromes.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2022Article
- The Red Edge: Bilin-Binding Photoreceptors as Optogenetic Tools and Fluorescence Reporters.Chemical reviews · 2021Review
- Structural basis of the protochromic green/red photocycle of the chromatic acclimation sensor RcaE.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Pressurized Liquid Extraction of a Phycocyanobilin Chromophore and Its Reconstitution with a Cyanobacteriochrome Photosensor for Efficient Isotopic Labeling.Plant & cell physiology · 2021Article
- Carbon Atoms Speaking Out: How the Geometric Sensitivity ofMolecules (Basel, Switzerland) · 2020Article
- Structural insights into photoactivation and signalling in plant phytochromes.Nature plants · 2020Article
- The primary structural photoresponse of phytochrome proteins captured by a femtosecond X-ray laser.eLife · 2020Article
- Protochromic absorption changes in the two-cysteine photocycle of a blue/orange cyanobacteriochrome.The Journal of biological chemistry · 2019Article
- MAS NMR on a Red/Far-Red Photochromic Cyanobacteriochrome All2699 fromInternational journal of molecular sciences · 2019Article
- 3D Structures of Plant Phytochrome A as Pr and Pfr From Solid-State NMR: Implications for Molecular Function.Frontiers in plant science · 2018Article
- The phycocyanobilin chromophore of streptophyte algal phytochromes is synthesized by HY2.The New phytologist · 2017Article
- NMR chemical shift pattern changed by ammonium sulfate precipitation in cyanobacterial phytochrome Cph1.Frontiers in molecular biosciences · 2015Article
- Phytochromes: an atomic perspective on photoactivation and signaling.The Plant cell · 2014Review
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phytochrome photoreceptors in plants and microorganisms switch photochromically between two states, controlling numerous important biological processes. Although this phototransformation is generally considered to involve rotation of ring D of the tetrapyrrole chromophore, Ulijasz et al. (Ulijasz, A. T., Cornilescu, G., Cornilescu, C. C., Zhang, J., Rivera, M., Markley, J. L., and Vierstra, R. D. (2010) Nature 463, 250-254) proposed that the A-ring rotates instead. Here, we apply magic angle spinning NMR to the two parent states following studies of the 23-kDa GAF (cGMP phosphodiesterase/adenylyl cyclase/FhlA) domain fragment of phytochrome from Synechococcus OS-B'. Major changes occur at the A-ring covalent linkage to the protein as well as at the protein residue contact of ring D. Conserved contacts associated with the A-ring nitrogen rule out an A-ring photoflip, whereas loss of contact of the D-ring nitrogen to the protein implies movement of ring D. Although none of the methine bridges showed a chemical shift change comparable with those characteristic of the D-ring photoflip in canonical phytochromes, denaturation experiments showed conclusively that the same occurs in Synechococcus OS-B' phytochrome upon photoconversion. The results are consistent with the D-ring being strongly tilted in both states and the C15=C16 double bond undergoing a Z/E isomerization upon light absorption. More subtle changes are associated with the A-ring linkage to the protein. Our findings thus disprove A-ring rotation and are discussed in relation to the position of the D-ring, photoisomerization, and photochromicity in the phytochrome family.
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