ArticleInvestigative ophthalmology & visual science2014
Swine cone and rod precursors arise sequentially and display sequential and transient integration and differentiation potential following transplantation.
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 35 citations in OpenAlex.
- Metabolic regulation of visual acuity.Science advances · 2025Article
- Robust generation of photoreceptor-dominant retinal organoids from porcine induced pluripotent stem cells.Stem cell reports · 2025Article
- Establishing the pig as a translational animal model for neurodevelopment.Translational neuroscience · 2025Article
- Retinal organoids with X-linked retinoschisis RS1 (E72K) mutation exhibit a photoreceptor developmental delay and are rescued by gene augmentation therapy.Stem cell research & therapy · 2024Article
- Pig Models in Retinal Research and Retinal Disease.Cold Spring Harbor perspectives in medicine · 2024Review
- Metabolic transcriptomics dictate responses of cone photoreceptors to retinitis pigmentosa.Cell reports · 2023Article
- Characterization of Green Fluorescent Protein in Heart Valves of a Transgenic Swine Model for Partial Heart Transplant Research.Journal of cardiovascular development and disease · 2023Article
- An experimental pig model with outer retinal degeneration induced by temporary intravitreal loading of N-methyl-N-nitrosourea during vitrectomy.Scientific reports · 2021Article
- Metabolic Deregulation of the Blood-Outer Retinal Barrier in Retinitis Pigmentosa.Cell reports · 2019Article
- Retinal disease in ciliopathies: Recent advances with a focus on stem cell-based therapies.Translational science of rare diseases · 2019Article
- Islet1 and Brn3 Expression Pattern Study in Human Retina and hiPSC-Derived Retinal Organoid.Stem cells international · 2019Article
- Correlation of spectral domain optical coherence tomography with histology and electron microscopy in the porcine retina.Experimental eye research · 2018Article
- Two-Step Reactivation of Dormant Cones in Retinitis Pigmentosa.Cell reports · 2016Article
- The Effect of Transient Local Anti-inflammatory Treatment on the Survival of Pig Retinal Progenitor Cell Allotransplants.Translational vision science & technology · 2015Article
- The potential of stem cell-based therapy for retinal repair.Neural regeneration research · 2014Article
- A Pro23His mutation alters prenatal rod photoreceptor morphology in a transgenic swine model of retinitis pigmentosa.Investigative ophthalmology & visual science · 2014Article
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
purposeWe followed cone and rod development in the pig and we correlated development with the potential for cone and rod precursor integration and differentiation following subretinal transplantation.
methodsRod and cone precursors were identified during development by their position in the outer retina and by immunostaining for markers of differentiation. Embryonic retinal cells from green fluorescent protein (GFP)(+) transgenic pigs at different developmental stages were transplanted into adult retinas and integration and differentiation was followed and quantified by immunostaining for markers of cone and rod differentiation.
resultsPig cones and rods are spatially segregated, allowing us to follow rod and cone development in situ. Gestation in the pig is 114 days. By embryonic day (E) 50, postmitotic cone progenitors had formed the outer two rows of the retina. These cone progenitors are marked by expression of Islet1 (ISL1) and Recoverin (RCVRN) (at this embryonic stage, RCVRN exclusively marks these cone precursors). By contrast, postmitotic neural retina leucine zipper (NRL)(+) rod precursors, located interior to the cone precursors, did not appear until E65. At E50, before NRL(+) rod precursors are evident, transplanted cells gave rise almost exclusively to cones. At, E57, transplanted cells gave rise to equal numbers of rods and cones, but by E65, transplanted cells gave rise almost exclusively to rods. Transplantation of cells at E85 or E105, as precursors initiate opsin expression, led to few integrated cells.
conclusionsConsistent with their sequential appearances in embryonic retina, these results demonstrate sequential and surprisingly narrow developmental windows for integration/differentiation of cone and rod precursors following transplantation.
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