Evidence map›Paper›PMID 24327095›Full record

ArticleArchives of virology2014

Molecular detection and genetic characterization of kobuviruses and astroviruses in asymptomatic local pigs in East Africa.

Joshua O Amimo, Edward Okoth, Joseph O Junga, William O Ogara, Moses N Njahira, Qiuhong Wang, Anastasia N Vlasova, Linda J Saif, Appolinaire Djikeng

Open access · bronzeAbstract read
In one paragraph

Article in Archives of virology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 48 citations in OpenAlex.

  1. Porcine astrovirus: a review.Archives of virology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Joshua O AmimoDepartment of Animal Production, Faculty of Veterinary Medicine, University of Nairobi, P.O Box 29053, Nairobi, 00625, Kenya, J.Amimo@cgiar.org.
Edward Okoth
Joseph O Junga
William O Ogara
Moses N Njahira
Qiuhong Wang
Anastasia N Vlasova
Linda J Saif
Appolinaire Djikeng
International Livestock Research Institute · KEThe Ohio State University · USUniversity of Nairobi · KE

Funding

Sustainable One Health Research Training Capacity: Molecular Epidemiology of Zoonotic Foodborne and Waterborne Pathogens in Eastern AfricaD43TW008650 · FIC · OHIO STATE UNIVERSITY · PI WONDWOSSEN A. GEBREYES, SAMUEL M KARIUKI · 2010 to 2026
$2.9M
FIC NIH HHS D43 TW008650
6 · The paper itself

Abstract

In this study, swine fecal specimens (n = 251) collected from nursing and weaned piglets raised under smallholder production systems were screened for the presence of kobuviruses by RT-PCR. Porcine kobuviruses were detected in 13.1 % (33/251) of the samples. We demonstrated that porcine kobuvirus infections exist in indigenous pigs in Kenya and Uganda and that the prevalence was higher in young piglets than older pigs: nursing piglets (15 %), post-weaning (3-month-old) pigs (17 %), 4-month-old pigs (10 %). Genetic analysis of the partial RNA-dependent RNA polymerase (RdRp) region (690 nt) revealed that kobuviruses circulating in East Africa are diverse, sharing nucleotide sequence identities ranging from 89.7 to 99.1 % and 88 to 92.3 % among them and with known porcine kobuviruses, respectively. The nucleotide sequence identities between our kobuvirus strains and those of human, bovine and canine kobuviruses were 69.4-70.7 %, 73.1-74.4 % and 67-70.7 %, respectively. Additionally, upon sequencing selected samples that showed consistent 720-bp RT-PCR bands while using the same primer set, we detected porcine astroviruses in our samples belonging to type 2 and type 3 mamastroviruses. To our knowledge, this study reports the first detection and molecular analysis of both porcine kobuviruses and astroviruses in an African region. Further studies are required to determine the role of these viruses in gastrointestinal infections of pigs in this region and to determine the genetic diversity of the circulating strains to develop accurate diagnostic tools and implement appropriate control strategies.

Indexed as

Africa, EasternAnimalsAstroviridaeAstroviridae InfectionsFecesGenetic VariationKobuvirusMolecular Sequence DataPicornaviridae InfectionsPrevalenceReverse Transcriptase Polymerase Chain ReactionRNA-Dependent RNA PolymeraseRNA, ViralSequence Analysis, DNASequence HomologySwineRNA-Dependent RNA PolymeraseRNA, Viral

Identifiers

PMID24327095
PMCPMC4094370
OpenAlexW2031751215

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.