ArticleJournal of visualized experiments : JoVE2013
An orthotopic bladder cancer model for gene delivery studies.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 9 citations in OpenAlex.
- Efficient Delivery of P3H4 siRNA and Chlorin e6 by cRGDfK-Installed Polyarginine Nanoparticles for Tumor-Targeting Therapy of Bladder Cancer.Pharmaceutics · 2022Article
- A Urinary Drug-Disposing Approach as an Alternative to Intravesical Chemotherapy for Treating Nonmuscle Invasive Bladder Cancer.Cancer research · 2022Article
- FimH confers mannose-targeting ability to Bacillus Calmette-Guerin for improved immunotherapy in bladder cancer.Journal for immunotherapy of cancer · 2022Article
- Methods to assess anticancer immune responses in orthotopic bladder carcinomas.Methods in enzymology · 2020Article
- Inhibition of Histone Deacetylase (HDAC) Enhances Checkpoint Blockade Efficacy by Rendering Bladder Cancer Cells Visible for T Cell-Mediated Destruction.Frontiers in oncology · 2020Article
- Expression of bladder cancer-associated glycans in murine tumor cell lines.Oncology letters · 2019Article
- Somatic mutation of the cohesin complex subunit confers therapeutic vulnerabilities in cancer.The Journal of clinical investigation · 2018Article
- In vivo biodistribution and toxicity of intravesical administration of quantum dots for optical molecular imaging of bladder cancer.Scientific reports · 2017Article
- A Murine Orthotopic Bladder Tumor Model and Tumor Detection System.Journal of visualized experiments : JoVE · 2017Article
- Polymer-enhanced delivery increases adenoviral gene expression in an orthotopic model of bladder cancer.Journal of controlled release : official journal of the Controlled Release Society · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Bladder cancer is the second most common cancer of the urogenital tract and novel therapeutic approaches that can reduce recurrence and progression are needed. The tumor microenvironment can significantly influence tumor development and therapy response. It is therefore often desirable to grow tumor cells in the organ from which they originated. This protocol describes an orthotopic model of bladder cancer, in which MB49 murine bladder carcinoma cells are instilled into the bladder via catheterization. Successful tumor cell implantation in this model requires disruption of the protective glycosaminoglycan layer, which can be accomplished by physical or chemical means. In our protocol the bladder is treated with trypsin prior to cell instillation. Catheterization of the bladder can also be used to deliver therapeutics once the tumors are established. This protocol describes the delivery of an adenoviral construct that expresses a luciferase reporter gene. While our protocol has been optimized for short-term studies and focuses on gene delivery, the methodology of mouse bladder catheterization has broad applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.