ArticleThe Plant cell2013
Phosphatidylinositol 4,5-bisphosphate influences PIN polarization by controlling clathrin-mediated membrane trafficking in Arabidopsis.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 162 citations in OpenAlex.
- Temporal GWAS and QTL mapping of UAV-derived digital canopy height reveals stage-specific genetic effects in bread wheat.Plant phenomics (Washington, D.C.) · 2026Article
- ATM2 promotes phosphoinositide formation to stabilize actin-plasma membrane contacts in pollen tubes.Nature plants · 2026Article
- The class VIII myosin ATM2 stabilizes actin-plasma membrane contacts in tobacco pollen tubes by locally promoting PtdIns(4,5)PNature plants · 2026Article
- Phospholipid Networks as Metabolic Hubs and Signaling Integrators in Plant Development and Stress Adaptation.Plants (Basel, Switzerland) · 2026Review
- Roles of rubber elongation factor and small rubber particle protein in rubber particles.Plant molecular biology · 2025Review
- Plant PI-PLC signaling in stress and development.Plant physiology · 2025Review
- A chemical genetic screen with the EXO70 inhibitor Endosidin2 uncovers potential modulators of exocytosis in Arabidopsis.Plant direct · 2024Article
- Ectopic assembly of an auxin efflux control machinery shifts developmental trajectories.The Plant cell · 2024Article
- Phosphoinositides in plant-pathogen interaction: trends and perspectives.Stress biology · 2023Review
- A phosphoinositide hub connects CLE peptide signaling and polar auxin efflux regulation.Nature communications · 2023Article
- Mitochondrial GPAT-derived LPA controls auxin-dependent embryonic and postembryonic development.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Bipartite phosphoinositide-dependent modulation of auxin signaling during xylem differentiation in Arabidopsis thaliana roots.The New phytologist · 2022Article
- Leveraging orthology within maize and Arabidopsis QTL to identify genes affecting natural variation in gravitropism.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- TheeLife · 2022Article
- Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components.The Plant cell · 2022Article
- An Arabidopsis mutant deficient in phosphatidylinositol-4-phosphate kinases ß1 and ß2 displays altered auxin-related responses in roots.Scientific reports · 2022Article
- Molecular mechanisms of endomembrane trafficking in plants.The Plant cell · 2022Review
- Subcellular trafficking and post-translational modification regulate PIN polarity in plants.Frontiers in plant science · 2022Review
- Dynamic apico-basal enrichment of the F-actin during cytokinesis in Arabidopsis cells embedded in their tissues.Quantitative plant biology · 2022Article
- Phospholipids in Salt Stress Response.Plants (Basel, Switzerland) · 2021Review
12 more citing papers are in PubMed but not listed here.
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Authors and funding
18 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The functions of the minor phospholipid phosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P2] during vegetative plant growth remain obscure. Here, we targeted two related phosphatidylinositol 4-phosphate 5-kinases (PI4P 5-kinases) PIP5K1 and PIP5K2, which are expressed ubiquitously in Arabidopsis thaliana. A pip5k1 pip5k2 double mutant with reduced PtdIns(4,5)P2 levels showed dwarf stature and phenotypes suggesting defects in auxin distribution. The roots of the pip5k1 pip5k2 double mutant had normal auxin levels but reduced auxin transport and altered distribution. Fluorescence-tagged auxin efflux carriers PIN-FORMED (PIN1)-green fluorescent protein (GFP) and PIN2-GFP displayed abnormal, partially apolar distribution. Furthermore, fewer brefeldin A-induced endosomal bodies decorated by PIN1-GFP or PIN2-GFP formed in pip5k1 pip5k2 mutants. Inducible overexpressor lines for PIP5K1 or PIP5K2 also exhibited phenotypes indicating misregulation of auxin-dependent processes, and immunolocalization showed reduced membrane association of PIN1 and PIN2. PIN cycling and polarization require clathrin-mediated endocytosis and labeled clathrin light chain also displayed altered localization patterns in the pip5k1 pip5k2 double mutant, consistent with a role for PtdIns(4,5)P2 in the regulation of clathrin-mediated endocytosis. Further biochemical tests on subcellular fractions enriched for clathrin-coated vesicles (CCVs) indicated that pip5k1 and pip5k2 mutants have reduced CCV-associated PI4P 5-kinase activity. Together, the data indicate an important role for PtdIns(4,5)P2 in the control of clathrin dynamics and in auxin distribution in Arabidopsis.
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