ArticleJournal of visualized experiments : JoVE2013
Dietary supplementation of polyunsaturated fatty acids in Caenorhabditis elegans.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- A defined microbial community remodels host metabolism and pathogen resistance inbioRxiv : the preprint server for biology · 2026Article
- Intestinal lipid metabolism controls molecular and behavioral immunity through NHR-68 and gut-brain signaling in Caenorhabditis elegans.PLoS biology · 2026Article
- Fasting requires Peroxiredoxin 2 and Peroxiredoxin 6 to coordinate redox dependent mitochondrial and lipid remodelling in Caenorhabditis elegans.Redox biology · 2026Article
- The Caenorhabditis elegans microbiome impacts microsporidia infection through nutrient limitation and spore inactivation.Nature communications · 2026Article
- A novel multisystem approach uncovers conserved mitochondrial complex I disease phenotypes and suppressors.Research square · 2026Article
- Developmental priming of adult proteostasis and longevity by NuA4 complex activity in early life.The EMBO journal · 2026Article
- Reductive death is averted by a conserved de novo lipogenic switch.Molecular cell · 2026Article
- Odd-Chain Fatty Acids-Enriched Algal Oil Improves Locomotor Function and Modulates Metabolic Pathways inMolecules (Basel, Switzerland) · 2026Article
- Reprograming gene expression in 'hibernating'eLife · 2025Article
- High Nutritional Conditions Influence Feeding Plasticity in Pristionchus pacificus and Render Worms Non-Predatory.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2025Article
- Ferroptosis inhibition by oleic acid mitigates iron-overload-induced injury.Cell chemical biology · 2024Article
- Ferroptosis precedes apoptosis to facilitate specific death signalling by fatty acids.Proceedings. Biological sciences · 2023Article
- Article
- Early-life starvation alters lipid metabolism in adults to cause developmental pathology in Caenorhabditis elegans.Genetics · 2023Article
- An antisteatosis response regulated by oleic acid through lipid droplet-mediated ERAD enhancement.Science advances · 2023Article
- Ether lipid deficiency disrupts lipid homeostasis leading to ferroptosis sensitivity.PLoS genetics · 2022Article
- Diet-responsive transcriptional regulation of insulin in a single neuron controls systemic metabolism.PLoS biology · 2022Article
- Stearoyl-CoA desaturases sustain cholinergic excitation and copulatory robustness in metabolically agingiScience · 2022Article
- Structured Long-Chain Omega-3 Fatty Acids for Improvement of Cognitive Function during Aging.International journal of molecular sciences · 2022Review
- Gene regulatory network inference in long-livediScience · 2022Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Fatty acids are essential for numerous cellular functions. They serve as efficient energy storage molecules, make up the hydrophobic core of membranes, and participate in various signaling pathways. Caenorhabditis elegans synthesizes all of the enzymes necessary to produce a range of omega-6 and omega-3 fatty acids. This, combined with the simple anatomy and range of available genetic tools, make it an attractive model to study fatty acid function. In order to investigate the genetic pathways that mediate the physiological effects of dietary fatty acids, we have developed a method to supplement the C. elegans diet with unsaturated fatty acids. Supplementation is an effective means to alter the fatty acid composition of worms and can also be used to rescue defects in fatty acid-deficient mutants. Our method uses nematode growth medium agar (NGM) supplemented with fatty acid sodium salts. The fatty acids in the supplemented plates become incorporated into the membranes of the bacterial food source, which is then taken up by the C. elegans that feed on the supplemented bacteria. We also describe a gas chromatography protocol to monitor the changes in fatty acid composition that occur in supplemented worms. This is an efficient way to supplement the diets of both large and small populations of C. elegans, allowing for a range of applications for this method.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.