Evidence map›Paper›PMID 24326392›Full record

ArticleJournal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism2014

Transplantation of neural stem cells that overexpress SOD1 enhances amelioration of intracerebral hemorrhage in mice.

Takuma Wakai, Hiroyuki Sakata, Purnima Narasimhan, Hideyuki Yoshioka, Hiroyuki Kinouchi, Pak H Chan

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed, 1 pooled it
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.

  1. Pooled it
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  7. Article
  8. Article
  9. EndogenousNeural regeneration research · 2022
    Review
  10. The Role of Copper Homeostasis in Brain Disease.International journal of molecular sciences · 2022
    Review
  11. Review
  12. Recent Stem Cell Research on Hemorrhagic Stroke : An Update.Journal of Korean Neurosurgical Society · 2022
    Article
  13. Article
  14. Article
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  16. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Takuma Wakai1] Department of Neurosurgery, Stanford University School of Medicine, Stanford, California, USA [2] Department of Neurology and Neurological Sciences and Program in Neurosciences, Stanford University School of Medicine, Stanford, California, USA.
Hiroyuki Sakata1] Department of Neurosurgery, Stanford University School of Medicine, Stanford, California, USA [2] Department of Neurology and Neurological Sciences and Program in Neurosciences, Stanford University School of Medicine, Stanford, California, USA.
Purnima Narasimhan1] Department of Neurosurgery, Stanford University School of Medicine, Stanford, California, USA [2] Department of Neurology and Neurological Sciences and Program in Neurosciences, Stanford University School of Medicine, Stanford, California, USA.
Hideyuki YoshiokaDepartment of Neurosurgery, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, Japan.
Hiroyuki KinouchiDepartment of Neurosurgery, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, Japan.
Pak H Chan1] Department of Neurosurgery, Stanford University School of Medicine, Stanford, California, USA [2] Department of Neurology and Neurological Sciences and Program in Neurosciences, Stanford University School of Medicine, Stanford, California, USA.
Stanford University · USUniversity of Yamanashi · JP

Funding

Reperfusion and Neurovascular Injury in StrokeR01NS025372 · NINDS · UNIVERSITY OF CALIFORNIA SAN FRANCISCO · PI CHAN, PAK H · 1988 to 2015
$5.0M
Cell Death/Survival After Global Cerebral IschemiaR01NS038653 · NINDS · STANFORD UNIVERSITY · PI CHAN, PAK H · 1998 to 2013
$4.5M
NINDS NIH HHS R01 NS025372NINDS NIH HHS R01 NS038653
6 · The paper itself

Abstract

Previous studies have shown that intraparenchymal transplantation of neural stem cells (NSCs) ameliorates neurologic deficits in animals with intracerebral hemorrhage (ICH). However, massive grafted cell death after transplantation, possibly caused by a hostile host brain environment, lessens the effectiveness of this approach. We focused on the effect of oxidative stress against grafted NSCs and hypothesized that conferring antioxidant properties to transplanted NSCs may overcome their death and enhance neuroprotection after ICH. Copper/zinc-superoxide dismutase (SOD1) is a specific antioxidant enzyme that counteracts superoxide anions. We investigated whether genetic manipulation to overexpress SOD1 enhances survival of grafted NSCs and accelerates amelioration of ICH. Neural stem cells that overexpress SOD1 were administered intracerebrally 3 days after ICH in a mouse model. Histologic and behavioral tests were examined after ICH. Copper/zinc-superoxide dismutase overexpression protected the grafted NSCs via a decrease in production of reactive oxygen species. This resulted in an increase in paracrine factors released by the NSCs, and an increase in surviving neurons in the striatum and a reduction in striatal atrophy. In addition, SOD1 overexpression showed progressive improvement in behavioral recovery. Our results suggest that enhanced antioxidative activity in NSCs improves efficacy of stem cell therapy for ICH.

Indexed as

AnimalsBehavior, AnimalBlotting, WesternCell Culture TechniquesCell DifferentiationCell SeparationCell SurvivalCerebral HemorrhageGreen Fluorescent ProteinsMiceMice, Inbred C57BLMice, TransgenicMicroscopy, FluorescenceNeural Stem CellsStem Cell TransplantationSuperoxide DismutaseGreen Fluorescent ProteinsSod1 protein, mouseSuperoxide DismutaseSuperoxide Dismutase-1Superoxides

Identifiers

PMID24326392
PMCPMC3948120
OpenAlexW2045292934

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.