Evidence map›Paper›PMID 24325873›Full record

ArticleParasite (Paris, France)2013

Expression and immunolocalisation of TpFABP as a candidate antigen for the serodiagnosis of rabbit Taenia pisiformis cysticercosis.

Deying Yang, Lin Chen, Yue Xie, Xuhang Wu, Xiang Nong, Xi Peng, Weimin Lai, Xiaobin Gu, Shuxian Wang, Xuerong Peng and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasite (Paris, France), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 85% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 8 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Deying YangDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Lin ChenDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Yue XieDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Xuhang WuDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Xiang NongDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Xi PengCollege of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Weimin LaiCollege of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Xiaobin GuDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Shuxian WangDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Xuerong PengCollege of Life and Basic Science, Sichuan Agricultural University, Ya'an 625014, China.
Guangyou YangDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Sichuan Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The larval stage of Taenia pisiformis, also known as Cysticercus pisiformis, is the causative agent of cysticercosis and the cause of severe health problems in rabbits that negatively impacts on husbandry production. To date, there is no fast detection method to identify early infections in rabbits. In the present study, a new dot-ELISA-based on an endogenous antigen fatty acid-binding protein (FABP) was developed for the detection of cysticercosis, and its potential was then evaluated using test serum samples. Immunolocalisation showed that T. pisiformis FABP (TpFABP) localised to the parenchyma of the bladder wall of the cysticercus and perinuclear cytoplasm of parenchyma of the adult parasite. After cloning and expression, recombinant TpFABP (rTpFABP) protein was used for serodiagnosis of T. pisiformis infection in rabbits by dot-ELISA. The antibody was detected 14 days post-infection in rabbits experimentally infected with T. pisiformis. Based on the necropsy results, the sensitivity and specificity of 169 serum samples tested by rTpFABP dot-ELISA were found to be 98.2% (54/55) and 92.1% (105/114), respectively. These data suggest that the dot-ELISA developed in this study has potential for detection of T. pisiformis infection in rabbits.

Indexed as

Amino Acid SequenceAnimalsAntibodies, HelminthAntigens, HelminthBlotting, WesternCysticercosisDNA, ComplementaryDNA, HelminthEnzyme-Linked Immunosorbent AssayFatty Acid-Binding ProteinsFemaleGene ExpressionImmune SeraMolecular Sequence DataRabbitsSequence AlignmentAntibodies, HelminthAntigens, HelminthDNA, ComplementaryDNA, HelminthFatty Acid-Binding ProteinsImmune Sera

Identifiers

PMID24325873
PMCPMC3859029
OpenAlexW2155506817

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.