ArticleStem cells and development2014
Novel and robust transplantation reveals the acquisition of polarized processes by cortical cells derived from mouse and human pluripotent stem cells.
Article in Stem cells and development, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Therapeutic Potential of Experimental Stereotactic Hippocampal Cell Transplant in the Management of Alzheimer's Disease.Journal of clinical medicine · 2025Review
- Synaptic neoteny of human cortical neurons requires species-specific balancing of SRGAP2-SYNGAP1 cross-inhibition.Neuron · 2024Article
- SYNGAP1 deficiency disrupts synaptic neoteny in xenotransplanted human cortical neurons in vivo.Neuron · 2024Article
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- Direct visualization of the transition status during neural differentiation by dual-fluorescent reporter human pluripotent stem cells.Stem cell reports · 2022Article
- Regulation of the Brain Neural Niche by Soluble Molecule Akhirin.Journal of developmental biology · 2021Review
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- Implications of Extended Inhibitory Neuron Development.International journal of molecular sciences · 2021Review
- Immune-tolerance to human iPS-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing.Scientific reports · 2021Article
- Article
- Article
- Concise Review: Human-Animal Neurological Chimeras: Humanized Animals or Human Cells in an Animal?Stem cells (Dayton, Ohio) · 2019Review
- Review
- Induced pluripotent stem cells as a tool to study brain circuits in autism-related disorders.Stem cell research & therapy · 2018Review
- Creating Patient-Specific Neural Cells for the In Vitro Study of Brain Disorders.Stem cell reports · 2015Article
- Sildenefil increases connexin 40 in smooth muscle cells through activation of BMP pathways in pulmonary arterial hypertension.International journal of clinical and experimental pathology · 2014Article
- Human Keratinocytes Adopt Neuronal Fates AfterCell transplantationArticle
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Current stem cell technologies have enabled the induction of cortical progenitors and neurons from embryonic stem cells (ESCs) and induced pluripotent stem cells in vitro. To understand the mechanisms underlying the acquisition of apico-basal polarity and the formation of processes associated with the stemness of cortical cells generated in monolayer culture, here, we developed a novel in utero transplantation system based on the moderate dissociation of adherens junctions in neuroepithelial tissue. This method enables (1) the incorporation of remarkably higher numbers of grafted cells and (2) quantitative morphological analyses at single-cell resolution, including time-lapse recording analyses. We then grafted cortical progenitors induced from mouse ESCs into the developing brain. Importantly, we revealed that the mode of process extension depends on the extrinsic apico-basal polarity of the host epithelial tissue, as well as on the intrinsic differentiation state of the grafted cells. Further, we successfully transplanted cortical progenitors induced from human ESCs, showing that our strategy enables investigation of the neurogenesis of human neural progenitors within the developing mouse cortex. Specifically, human cortical cells exhibit multiple features of radial migration. The robust transplantation method established here could be utilized both to uncover the missing gap between neurogenesis from ESCs and the tissue environment and as an in vivo model of normal and pathological human corticogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.