ArticleOptical nanoscopy2013
Resolving the spatial relationship between intracellular components by dual color super resolution optical fluctuations imaging (SOFI).
Article in Optical nanoscopy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- Fluorescence fluctuation-based super-resolution microscopy: Basic concepts for an easy start.Journal of microscopy · 2022Review
- Quantum Dots for Improved Single-Molecule Localization Microscopy.The journal of physical chemistry. B · 2021Article
- Spectral cross-cumulants for multicolor super-resolved SOFI imaging.Nature communications · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundMulti-color super-resolution (SR) imaging microscopy techniques can resolve ultrastructura relationships between- and provide co-localization information of- different proteins inside the cell or even within organelles at a higher resolution than afforded by conventional diffraction-limited imaging. While still very challenging, important SR colocalization results have been reported in recent years using STED, PALM and STORM techniques.
resultsIn this work, we demonstrate dual-color Super Resolution Optical Fluctuations Imaging (SOFI) using a standard far-field fluorescence microscope and different color blinking quantum dots. We define the spatial relationship between hDcp1a, a processing body (P-body, PB) protein, and the tubulin cytoskeletal network. Our finding could open up new perspectives on the role of the cytoskeleton in PB formation and assembly. Further insights into PB internal organization are also reported and discussed.
conclusionsOur results demonstrate the suitability and facile use of multi-color SOFI for the investigation of intracellular ultrastructures.
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