ArticleFrontiers in plant science2013
Unleashing the potential of the root hair cell as a single plant cell type model in root systems biology.
Article in Frontiers in plant science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- Genome wide association analysis of root hair traits in rice reveals novel genomic regions controlling epidermal cell differentiation.BMC plant biology · 2023Article
- Identification of new marker genes from plant single-cell RNA-seq data using interpretable machine learning methods.The New phytologist · 2022Article
- Root phenotypes as modulators of microbial microhabitats.Frontiers in plant science · 2022Review
- Expanding the application of a UV-visible reporter for transient gene expression and stable transformation in plants.Horticulture research · 2021Article
- A starting guide to root ecology: strengthening ecological concepts and standardising root classification, sampling, processing and trait measurements.The New phytologist · 2021Review
- Expression Partitioning of Duplicate Genes at Single Cell Resolution inFrontiers in genetics · 2020Article
- Article
- Transcriptomics of Arabidopsis sperm cells at single-cell resolution.Plant reproduction · 2019Article
- Root Hair Single Cell Type Specific Profiles of Gene Expression and Alternative Polyadenylation Under Cadmium Stress.Frontiers in plant science · 2019Article
- A comparative genomic and transcriptomic analysis at the level of isolated root hair cells reveals new conserved root hair regulatory elements.Plant molecular biology · 2017Article
- Diversification of Root Hair Development Genes in Vascular Plants.Plant physiology · 2017Article
- Laser microdissection of tomato fruit cell and tissue types for transcriptome profiling.Nature protocols · 2016Article
- Decipher the Molecular Response of Plant Single Cell Types to Environmental Stresses.BioMed research international · 2016Review
- A Comparative Epigenomic Analysis of Polyploidy-Derived Genes in Soybean and Common Bean.Plant physiology · 2015Article
- Conserved Gene Expression Programs in Developing Roots from Diverse Plants.The Plant cell · 2015Article
- Molecular phenotyping of plant single cell-types enhances forward genetic analyses.Frontiers in plant science · 2015Article
- The female gametophyte: an emerging model for cell type-specific systems biology in plant development.Frontiers in plant science · 2015Review
- System approaches to study root hairs as a single cell plant model: current status and future perspectives.Frontiers in plant science · 2015Review
- Root systems biology.Frontiers in plant science · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant root is an organ composed of multiple cell types with different functions. This multicellular complexity limits our understanding of root biology because -omics studies performed at the level of the entire root reflect the average responses of all cells composing the organ. To overcome this difficulty and allow a more comprehensive understanding of root cell biology, an approach is needed that would focus on one single cell type in the plant root. Because of its biological functions (i.e., uptake of water and various nutrients; primary site of infection by nitrogen-fixing bacteria in legumes), the root hair cell is an attractive single cell model to study root cell response to various stresses and treatments. To fully study their biology, we have recently optimized procedures in obtaining root hair cell samples. We culture the plants using an ultrasound aeroponic system maximizing root hair cell density on the entire root systems and allowing the homogeneous treatment of the root system. We then isolate the root hair cells in liquid nitrogen. Isolated root hair yields could be up to 800 to 1000~mg of plant cells from 60 root systems. Using soybean as a model, the purity of the root hair was assessed by comparing the expression level of genes previously identified as soybean root hair specific between preparations of isolated root hair cells and stripped roots, roots devoid in root hairs. Enlarging our tests to include other plant species, our results support the isolation of large quantities of highly purified root hair cells which is compatible with a systems biology approach.
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