ArticleProceedings of the National Academy of Sciences of the United States of America2013
HPr antagonizes the anti-σ70 activity of Rsd in Escherichia coli.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 51 citations in OpenAlex.
- The phosphoenolpyruvate phosphotransferase system regulates cell length and width through semi-independent pathways.Molecular biology of the cell · 2026Article
- Transcriptional and Metabolic Response of a Strain ofBiotech (Basel (Switzerland)) · 2024Article
- RpoS and the bacterial general stress response.Microbiology and molecular biology reviews : MMBR · 2024Review
- Awakening the natural capability of psicose production in Escherichia coli.NPJ science of food · 2023Article
- How Bacterial Pathogens Coordinate Appetite with Virulence.Microbiology and molecular biology reviews : MMBR · 2023Review
- HPr prevents FruR-mediated facilitation of RNA polymerase binding to the fru promoter in Vibrio cholerae.Nucleic acids research · 2023Article
- Discovery of AI-2 Quorum Sensing Inhibitors Targeting the LsrK/HPr Protein-Protein Interaction Site by Molecular Dynamics Simulation, Virtual Screening, and Bioassay Evaluation.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Growth ofJournal of oral microbiology · 2023Article
- Cortisol Promotes Surface Translocation ofPathogens (Basel, Switzerland) · 2022Article
- Residual Helicity at the Active Site of the Histidine Phosphocarrier, HPr, Modulates Binding Affinity to Its Natural Partners.International journal of molecular sciences · 2021Article
- The RelA hydrolase domain acts as a molecular switch for (p)ppGpp synthesis.Communications biology · 2021Article
- A Novel Regulation of K-antigen Capsule Synthesis inFrontiers in oral health · 2021Article
- Distinct Amino Acid Availability-Dependent Regulatory Mechanisms of MepS and MepM Levels inFrontiers in microbiology · 2021Article
- Synthesis of ppGpp impacts type IX secretion and biofilm matrix formation in Porphyromonas gingivalis.NPJ biofilms and microbiomes · 2020Article
- Sugar-mediated regulation of a c-di-GMP phosphodiesterase in Vibrio cholerae.Nature communications · 2019Article
- Structural insight into glucose repression of the mannitol operon.Scientific reports · 2019Article
- Make and break the alarmone: regulation of (p)ppGpp synthetase/hydrolase enzymes in bacteria.FEMS microbiology reviews · 2019Review
- Diversity, versatility and complexity of bacterial gene regulation mechanisms: opportunities and drawbacks for applications in synthetic biology.FEMS microbiology reviews · 2019Review
- Determination of protein phosphorylation by polyacrylamide gel electrophoresis.Journal of microbiology (Seoul, Korea) · 2019Article
- Regulation of (p)ppGpp hydrolysis by a conserved archetypal regulatory domain.Nucleic acids research · 2019Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bacterial phosphoenolpyruvate:sugar phosphotransferase system (PTS) is a multicomponent system that participates in a variety of physiological processes in addition to the phosphorylation-coupled transport of numerous sugars. In Escherichia coli and other enteric bacteria, enzyme IIA(Glc) (EIIA(Glc)) is known as the central processing unit of carbon metabolism and plays multiple roles, including regulation of adenylyl cyclase, the fermentation/respiration switch protein FrsA, glycerol kinase, and several non-PTS transporters, whereas the only known regulatory role of the E. coli histidine-containing phosphocarrier protein HPr is in the activation of glycogen phosphorylase. Because HPr is known to be more abundant than EIIA(Glc) in enteric bacteria, we assumed that there might be more regulatory mechanisms connected with HPr. The ligand fishing experiment in this study identified Rsd, an anti-sigma factor known to complex with σ(70) in stationary-phase cells, as an HPr-binding protein in E. coli. Only the dephosphorylated form of HPr formed a tight complex with Rsd and thereby inhibited complex formation between Rsd and σ(70). Dephosphorylated HPr, but not phosphorylated HPr, antagonized the inhibitory effect of Rsd on σ(70)-dependent transcriptions both in vivo and in vitro, and also influenced the competition between σ(70) and σ(S) for core RNA polymerase in the presence of Rsd. Based on these data, we propose that the anti-σ(70) activity of Rsd is regulated by the phosphorylation state-dependent interaction of HPr with Rsd.
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