ArticleBiotechnology journal2014
In Pichia pastoris, growth rate regulates protein synthesis and secretion, mating and stress response.
Article in Biotechnology journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 107 citations in OpenAlex.
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- Fed-Batch Operational Strategies for Efficient and Robust Pichia Pastoris Cultivations Achieving High Cell Densities.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Establishing Reference Genes for Pichia pastoris Quantitative Real-Time Polymerase Chain Reaction Analyses.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Uncoupling protein production from growth: different strategies for intracellular and secreted proteins in yeast.Microbial cell factories · 2025Article
- The role of ATP citrate lyase, phosphoketolase, and malic enzyme in oleaginous Rhodotorula toruloides.Applied microbiology and biotechnology · 2025Article
- Chances and drawbacks of derepressed recombinant enzyme production in continuous cultivations withFrontiers in bioengineering and biotechnology · 2025Article
- Metabolic Engineering ofMolecules (Basel, Switzerland) · 2024Article
- Recombinant production of Paenibacillus wynnii β-galactosidase with Komagataella phaffii.Microbial cell factories · 2024Article
- Evaluation of different glycerol fed-batch strategies in a lab-scale bioreactor for the improved production of a novel engineered β-fructofuranosidase enzyme in Pichia pastoris.World journal of microbiology & biotechnology · 2024Article
- Protein production dynamics and physiological adaptation of recombinant Komagataella phaffii at near-zero growth rates.Microbial cell factories · 2024Article
- Synergic kinetic and physiological control to improve the efficiency of Komagataella phaffii recombinant protein production bioprocesses.Microbial biotechnology · 2024Article
- Characterising the metabolic rewiring of extremely slow growing Komagataella phaffii.Microbial biotechnology · 2024Article
- Pichia pastoris growth-coupled heme biosynthesis analysis using metabolic modelling.Scientific reports · 2023Article
- Is heterogeneity in large-scale bioreactors a real problem in recombinant protein synthesis by Pichia pastoris?Applied microbiology and biotechnology · 2023Article
- Industrial Production of Proteins withBiomolecules · 2023Review
- Cofactor Engineering for Efficient Production of α-Farnesene by Rational Modification of NADPH and ATP Regeneration Pathway inInternational journal of molecular sciences · 2023Article
- Production, purification, and characterization of recombinant rabies virus glycoprotein expressed in PichiaPink™ yeast.Biotechnology reports (Amsterdam, Netherlands) · 2022Article
- Article
- What makes Komagataella phaffii non-conventional?FEMS yeast research · 2021Review
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Protein production in yeasts is related to the specific growth rate μ. To elucidate on this correlation, we studied the transcriptome of Pichia pastoris at different specific growth rates by cultivating a strain secreting human serum albumin at μ = 0.015 to 0.15 h(-1) in glucose-limited chemostats. Genome-wide regulation revealed that translation-related as well as mitochondrial genes were upregulated with increasing μ, while autophagy and other proteolytic processes, carbon source-responsive genes and other targets of the TOR pathway as well as many transcriptional regulators were downregulated at higher μ. Mating and sporulation genes were most active at intermediate μ of 0.05 and 0.075 h(-1) . At very slow growth (μ = 0.015 h(-1) ) gene regulation differs significantly, affecting many transporters and glucose sensing. Analysis of a subset of genes related to protein folding and secretion reveals that unfolded protein response targets such as translocation, endoplasmic reticulum genes, and cytosolic chaperones are upregulated with increasing growth rate while proteolytic degradation of secretory proteins is downregulated. We conclude that a high μ positively affects specific protein secretion rates by acting on multiple cellular processes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.