ArticleMolecular and cellular biology1986
Nonmuscle and muscle tropomyosin isoforms are expressed from a single gene by alternative RNA splicing and polyadenylation.
Article in Molecular and cellular biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed, 192 citations in OpenAlex.
- Elucidating the coordination of RNA processing using short-read and long-read RNA-sequencing methods.Nature reviews. Molecular cell biology · 2026Review
- Molecular cloning ofAnimal biotechnology · 2024Article
- Nanopore sequencing reveals full-length Tropomyosin 1 isoforms and their regulation by RNA-binding proteins during rat heart development.Journal of cellular and molecular medicine · 2021Article
- LncRNA WEE2-AS1 knockdown inhibits the proliferation, migration and invasion of glioma cells via regulating miR-29b-2-5p/TPM3 axis.Oncology research · 2021Article
- Full-length transcriptome reconstruction reveals a large diversity of RNA and protein isoforms in rat hippocampus.Nature communications · 2019Article
- Getting the Entire Message: Progress in Isoform Sequencing.Frontiers in genetics · 2019Review
- Microfluidic isoform sequencing shows widespread splicing coordination in the human transcriptome.Genome research · 2018Article
- Differential gene expression profiling of mouse uterine luminal epithelium during periimplantation.Reproductive sciences (Thousand Oaks, Calif.) · 2014Article
- Tropomodulins: pointed-end capping proteins that regulate actin filament architecture in diverse cell types.Cytoskeleton (Hoboken, N.J.) · 2012Review
- Tropomodulin isoforms regulate thin filament pointed-end capping and skeletal muscle physiology.The Journal of cell biology · 2010Article
- New insights into the regulation of the actin cytoskeleton by tropomyosin.International review of cell and molecular biology · 2010Review
- Proteomic studies of rat tibialis anterior muscle during postnatal growth and development.Molecular and cellular biochemistry · 2009Article
- Investigation of differentially expressed proteins in rat gastrocnemius muscle during denervation-reinnervation.Journal of muscle research and cell motility · 2006Article
- Structure and tropomyosin binding properties of the N-terminal capping domain of tropomodulin 1.Biophysical journal · 2005Article
- Charged residue changes in the carboxy-terminus of alpha-tropomyosin alter mouse cardiac muscle contractility.The Journal of physiology · 2004Article
- Gamma tropomyosin gene products are required for embryonic development.Molecular and cellular biology · 2004Article
- Overexpression of kinase suppressor of Ras upregulates the high-molecular-weight tropomyosin isoforms in ras-transformed NIH 3T3 fibroblasts.Molecular and cellular biology · 2003Article
- Tropomyosin requires an intact N-terminal coiled coil to interact with tropomodulin.Biophysical journal · 2002Article
- The PDZ domain of the LIM protein enigma binds to beta-tropomyosin.Molecular biology of the cell · 1999Article
- Exonic splicing enhancers contribute to the use of both 3' and 5' splice site usage of rat beta-tropomyosin pre-mRNA.RNA (New York, N.Y.) · 1999Article
8 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The molecular basis for the expression of rat embryonic fibroblast tropomyosin 1 and skeletal muscle beta-tropomyosin was determined. cDNA clones encoding these tropomyosin isoforms exhibit complete identity except for two carboxy-proximal regions (amino acids 189 to 213 and 258 to 284) and different 3'-untranslated sequences. The isoform-specific regions delineate the troponin T-binding domains of skeletal muscle tropomyosin. Analysis of genomic clones indicates that there are two separate loci in the rat genome that contain sequences complementary to these mRNAs. One locus is a pseudogene. The other locus contains a single gene made up of 11 exons and spans approximately 10 kilobases. Sequences common to all mRNAs were found in exons 1 through 5 (amino acids 1 to 188) and exons 8 and 9 (amino acids 214 to 257). Exons 6 and 11 are specific for fibroblast mRNA (amino acids 189 to 213 and 258 to 284, respectively), while exons 7 and 10 are specific for skeletal muscle mRNA (amino acids 189 to 213 and 258 to 284, respectively). In addition, exons 10 and 11 each contain the entire 3'-untranslated sequences of the respective mRNAs including the polyadenylation site. Although the gene is also expressed in smooth muscle (stomach, uterus, and vas deferens), only the fibroblast-type splice products can be detected in these tissues. S1 and primer extension analyses indicate that all mRNAs expressed from this gene are transcribed from a single promoter. The promoter was found to contain G-C-rich sequences, a TATA-like sequence TTTTA, no identifiable CCAAT box, and two putative Sp1-binding sites.
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