Evidence map›Paper›PMID 24323415›Full record

ArticleTranslational stroke research2013

mRNA redistribution during permanent focal cerebral ischemia.

Monique K Lewis, Jill T Jamison, Joseph C Dunbar, Donald J DeGracia

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Translational stroke research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 90% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Monique K LewisDepartment of Physiology, Wayne State University School of Medicine, 4116 Scott Hall, 540 East Canfield Ave, Detroit, MI, 48201, USA.
Jill T Jamison
Joseph C Dunbar
Donald J DeGracia
Wayne State University · US

Funding

THE ROLE OF CNS INSULIN AS A MODULATOR OF DIABETIC COMPLICATIONSR25GM058905 · NIGMS · WAYNE STATE UNIVERSITY · PI CHOW, CHRISTINE S, DUNBAR, JOSEPH C · 1999 to 2020
$14.4M
Stress Granules Cause Translation Arrest In Ischemic Vulunerable NeuronsR01NS057167 · NINDS · WAYNE STATE UNIVERSITY · PI DEGRACIA, DONALD J · 2008 to 2012
$1.7M
NIGMS NIH HHS GM58905-8NIGMS NIH HHS R25 GM058905NINDS NIH HHS NS057167NINDS NIH HHS R01 NS057167
6 · The paper itself

Abstract

Translation arrest occurs in neurons following focal cerebral ischemia and is irreversible in penumbral neurons destined to die. Following global cerebral ischemia, mRNA is sequestered away from 40S ribosomal subunits as mRNA granules, precluding translation. Here, we investigated mRNA granule formation using fluorescence in situ histochemistry out to 8 h permanent focal cerebral ischemia using middle cerebral artery occlusion in Long Evans rats with and without diabetes. Neuronal mRNA granules colocalized with PABP, HuR, and NeuN, but not 40S or 60S ribosomal subunits, or organelle markers. The volume of brain with mRNA granule-containing neurons decreased exponentially with ischemia duration, and was zero after 8 h permanent focal cerebral ischemia or any duration of ischemia in diabetic rats. These results show that neuronal mRNA granule response has a limited range of insult intensity over which it is expressed. Identifying the limits of effective neuronal stress response to ischemia will be important for developing effective stroke therapies.

Indexed as

AnimalsAntigens, NuclearBrain IschemiaDiabetes Mellitus, ExperimentalELAV ProteinsInfarction, Middle Cerebral ArteryIn Situ Hybridization, FluorescenceMaleNerve Tissue ProteinsNeuronsPoly(A)-Binding ProteinsRatsRats, Long-EvansRibosome Subunits, Large, EukaryoticRibosome Subunits, Small, EukaryoticRNA, MessengerAntigens, NuclearELAV ProteinsNerve Tissue ProteinsPoly(A)-Binding ProteinsRbfox3 protein, ratRNA, Messenger

Identifiers

PMID24323415
PMCPMC3864703
OpenAlexW2088847833

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.