ArticleBiotechnology letters2014
Production of tropane alkaloids in Hyoscyamus niger (black henbane) hairy roots grown in bubble-column and spray bioreactors.
Article in Biotechnology letters, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 55 citations in OpenAlex.
- Adventitious and Hairy Root Cultures ofInternational journal of molecular sciences · 2025Article
- Psychoactive Plant Database: a phytochemical resource for neurological drug discovery.Frontiers in pharmacology · 2025Article
- The Untapped Potential of Hairy Root Cultures and Their Multiple Applications.International journal of molecular sciences · 2024Review
- Biotechnological strategies for controlled accumulation of flavones in hairy root culture of Scutellaria lateriflora L.Scientific reports · 2023Article
- Metabolic Effects of Elicitors on the Biosynthesis of Tropane Alkaloids in Medicinal Plants.Plants (Basel, Switzerland) · 2023Review
- Hairy root culture: a potent method for improved secondary metabolite production of Solanaceous plants.Frontiers in plant science · 2023Review
- Design of nutrient gas-phase bioreactors: a critical comprehensive review.Bioprocess and biosystems engineering · 2022Review
- A novel spray bioreactor for the proliferation of plant callus; Hyoscyamus niger and Arnebia pulchra.Biotechnology letters · 2022Article
- Jasmonates in plant growth and development and elicitation of secondary metabolites: An updated overview.Frontiers in plant science · 2022Review
- Tropane Alkaloids: Chemistry, Pharmacology, Biosynthesis and Production.Molecules (Basel, Switzerland) · 2019Review
- Production of biomass and flavonoid ofJournal, genetic engineering & biotechnology · 2018Article
- Phenolic Compounds in Honey and Their Associated Health Benefits: A Review.Molecules (Basel, Switzerland) · 2018Review
- Improved production of dibenzocyclooctadiene lignans in the elicited microshoot cultures of Schisandra chinensis (Chinese magnolia vine).Applied microbiology and biotechnology · 2018Article
- Accumulation of dibenzocyclooctadiene lignans in agar cultures and in stationary and agitated liquid cultures of Schisandra chinensis (Turcz.) Baill.Applied microbiology and biotechnology · 2016Article
- Production of therapeutically relevant indolizidine alkaloids in Securinega suffruticosa in vitro shoots maintained in liquid culture systems.Applied biochemistry and biotechnology · 2015Article
- Enhanced production of hyoscyamine and scopolamine from genetically transformed root culture ofIn vitro cellular & developmental biology. Plant : journal of the Tissue Culture AssociationArticle
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hairy root cultures of Hyoscyamus niger were cultivated in shake-flasks, a bubble-column bioreactor and a hybrid bubble-column/spray bioreactor and evaluated for alkaloid production. The latter gave the highest anisodamine content (0.67 mg/g dry wt) whereas scopolamine, hyoscyamine and cuscohygrine concentrations were highest in the bubble-column reactor (5.3, 1.6 and 26.5 mg/g dry wt, respectively). Both bioreactors gave similar productivities of scopolamine (1 and 0.98 mg/l day) and cuscohygrine (5 and 5.4 mg/l day), but anisodamine productivity was 3.5-fold higher in the hybrid bioreactor (HB) (0.02 and 0.07 mg/l day, respectively). Elicitation with methyl jasmonate increased scopolamine productivity by 146 % in roots grown in the HB whereas their permeabilization with DMSO caused 4-, 5-, 25- and 28-fold increase in scopolamine, hyoscyamine, anisodamine and cuscohygrine concentrations in the growth medium. In situ extraction with Amberlite XAD-2 doubled scopolamine productivity in the hybrid reactor after 50 days.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.