ArticleGM crops & food
The wheat HMW-glutenin 1Dy10 gene promoter controls endosperm expression in Brachypodium distachyon.
Article in GM crops & food. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- CRISPR-Cas9 Gene Editing of thePlants (Basel, Switzerland) · 2022Article
- Developing specific leaf promoters tools for genetic use in transgenic plants towards food security.Saudi journal of biological sciences · 2021Article
- In Vitro Tissue Culture inInternational journal of molecular sciences · 2020Review
- Analyzing the action of evolutionarily conserved modules on HMW-GS 1Ax1 promoter activity.Plant molecular biology · 2020Article
- Over-expression of the Brachypodium ASR gene, BdASR4, enhances drought tolerance in Brachypodium distachyon.Plant cell reports · 2019Article
- Matrix association region/scaffold attachment region: the crucial player in defining the positions of chromosome breaks mediated by bile acid-induced apoptosis in nasopharyngeal epithelial cells.BMC medical genomics · 2019Article
- Matrix association region/scaffold attachment region (MAR/SAR) sequence: its vital role in mediating chromosome breakages in nasopharyngeal epithelial cells via oxidative stress-induced apoptosis.BMC molecular biology · 2018Article
- Identification of Leaf Promoters for Use in Transgenic Wheat.Plants (Basel, Switzerland) · 2018Article
- Investigating Triticeae anther gene promoter activity in transgenic Brachypodium distachyon.Planta · 2017Article
- BdCESA7, BdCESA8, and BdPMT Utility Promoter Constructs for Targeted Expression to Secondary Cell-Wall-Forming Cells of Grasses.Frontiers in plant science · 2016Article
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4 authors.
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Abstract
The grass species Brachypodium distachyon has emerged as a model system for the study of gene structure and function in temperate cereals. As a first demonstration of the utility of Brachypodium to study wheat gene promoter function, we transformed it with a T-DNA that included the uidA reporter gene under control of a wheat High-Molecular-Weight Glutenin Subunit (HMW-GS) gene promoter and transcription terminator. For comparison, the same expression cassette was introduced into wheat by biolistics. Histochemical staining for β-glucuronidase (GUS) activity showed that the wheat promoter was highly expressed in the endosperms of all the seeds of Brachypodium and wheat homozygous plants. It was not active in any other tissue of transgenic wheat, but showed variable and sporadic activity in a minority of styles of the pistils of four homozygous transgenic Brachypodium lines. The ease of obtaining transgenic Brachypodium plants and the overall faithfulness of expression of the wheat HMW-GS promoter in those plants make it likely that this model system can be used for studies of other promoters from cereal crop species that are difficult to transform.
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