ArticleScientific reports2013
Rapid formation of multicellular spheroids in double-emulsion droplets with controllable microenvironment.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed.
- Design and Synthesis of Fluorosurfactants for Microfluidic Droplet-Based Bioapplications.Polymer science & technology (Washington, D.C.) · 2026Review
- The application of experimental models for the drug discovery for digestive tumors.Molecular cancer · 2026Review
- Vascularized platforms for investigating cell communication via extracellular vesicles.Biomicrofluidics · 2024Review
- Scaffold-free development of multicellular tumor spheroids with spatial characterization of structure and metabolic radial profiles.In vitro models · 2024Article
- A Novel Multi-compartment Rotating Bioreactor for Improving ADSC-Spheroid Formation and its Application in Neurogenic Erectile Dysfunction.Current stem cell research & therapy · 2024Article
- Improved Neural Inductivity of Size-Controlled 3D Human Embryonic Stem Cells Using Magnetic Nanoparticles.Biomaterials research · 2024Article
- 3D Printing of Bioactive Gel-like Double Emulsion into a Biocompatible Hierarchical Macroporous Self-Lubricating Scaffold for 3D Cell Culture.ACS applied materials & interfaces · 2023Article
- Effect of Flow Rate Modulation on Alginate Emulsification in Multistage Microfluidics.Micromachines · 2023Article
- Comparison of Tumour-Specific Phenotypes in Human Primary and Expandable Pancreatic Cancer Cell Lines.International journal of molecular sciences · 2023Article
- Macrophage Profiling in Head and Neck Cancer to Improve Patient Prognosis and Assessment of Cancer Cell-Macrophage Interactions Using Three-Dimensional Coculture Models.International journal of molecular sciences · 2023Article
- Acoustic and Magnetic Stimuli-Based Three-Dimensional Cell Culture Platform for Tissue Engineering.Tissue engineering and regenerative medicine · 2023Review
- Recent Advances on Cell Culture Platforms for In Vitro Drug Screening and Cell Therapies: From Conventional to Microfluidic Strategies.Advanced healthcare materials · 2023Review
- Mesenchymal stromal cells for bone trauma, defects, and disease: Considerations for manufacturing, clinical translation, and effective treatments.Bone reports · 2023Article
- Spheroid Engineering in Microfluidic Devices.ACS omega · 2023Review
- Middle-out methods for spatiotemporal tissue engineering of organoids.Nature reviews bioengineering · 2023Review
- Comparison of EMT-Related and Multi-Drug Resistant Gene Expression, Extracellular Matrix Production, and Drug Sensitivity in NSCLC Spheroids Generated by Scaffold-Free and Scaffold-Based Methods.International journal of molecular sciences · 2022Article
- A Portable, Negative-Pressure Actuated, Dynamically Tunable Microfluidic Droplet Generator.Micromachines · 2022Article
- Engineering Organ-on-a-Chip to Accelerate Translational Research.Micromachines · 2022Review
- Microencapsulation-based cell therapies.Cellular and molecular life sciences : CMLS · 2022Review
- Multiparametric Material Functionality of Microtissue-Based In Vitro Models as Alternatives to Animal Testing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
22 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
An attractive option for tissue engineering is to use of multicellular spheroids as microtissues, particularly with stem cell spheroids. Conventional approaches of fabricating spheroids suffer from low throughput and polydispersity in size, and fail to supplement cues from extracellular matrix (ECM) for enhanced differentiation. In this study, we report the application of microfluidics-generated water-in-oil-in-water (w/o/w) double-emulsion (DE) droplets as pico-liter sized bioreactor for rapid cell assembly and well-controlled microenvironment for spheroid culture. Cells aggregated to form size-controllable (30-80 μm) spheroids in DE droplets within 150 min and could be retrieved via a droplet-releasing agent. Moreover, precursor hydrogel solution can be adopted as the inner phase to produce spheroid-encapsulated microgels after spheroid formation. As an example, the encapsulation of human mesenchymal stem cells (hMSC) spheroids in alginate and alginate-arginine-glycine-aspartic acid (-RGD) microgel was demonstrated, with enhanced osteogenic differentiation further exhibited in the latter case.
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Registered trials
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