Evidence map›Paper›PMID 24321511›Full record

ArticleNeuroscience2014

N-terminus regulation of VMAT2 mediates methamphetamine-stimulated efflux.

B Torres, A E Ruoho

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

B TorresUniversity of Wisconsin, Madison, Department of Neuroscience, 1300 University Avenue, Madison, WI 53706, USA.
A E RuohoUniversity of Wisconsin, Madison, Department of Neuroscience, 1300 University Avenue, Madison, WI 53706, USA. Electronic address: aeruoho@wisc.edu.
University of Wisconsin–Madison · US

Funding

Function of the Sigma-1 Receptor in MotoneuronsR21NS075820 · NINDS · UNIVERSITY OF WISCONSIN-MADISON · PI RUOHO, ARNOLD EINO · 2012 to 2013
$407k
NINDS NIH HHS R21 NS075820
6 · The paper itself

Abstract

The 20 amino acid (AA) N-terminus of the vesicular monoamine transporter 2 (VMAT2) was examined as a regulator of VMAT2 function. Removal of the first 16 or 19 AAs of the N-terminus resulted in a molecule with reduced ability to sequester [(3)H]-5HT. A glutathione-S-transferase-construct of the N-terminus underwent phosphorylation in the presence of PKC at serines 15 and 18. These putative phosphorylation sites were examined for effects on function. Phospho-mimetic substitution of serines 15 and 18 with aspartate in the full-length VMAT2 resulted in reduced [(3)H]-5HT sequestration and reduced methamphetamine (METH)-stimulated efflux of preloaded [(3)H]-5HT. In contrast, mutation of serines 15 and 18 to alanines maintained intact net substrate sequestration but eliminated METH-stimulated efflux of pre-accumulated [(3)H]-5HT. In summary, these data suggest a model in which the VMAT2 N-terminus regulates monoamine sequestration.

Indexed as

Adrenergic Uptake InhibitorsAnimalsCell LineCentral Nervous System StimulantsChlorocebus aethiopsCOS CellsEnzyme-Linked Immunosorbent AssayMethamphetamineModels, BiologicalMutationNeuroblastomaPhosphorylationSerotoninTetrabenazineTransfectionTritiumAdrenergic Uptake InhibitorsCentral Nervous System StimulantsMethamphetamineSerotoninTetrabenazineTritiumVesicular Monoamine Transport Proteins[(3)H]-tetrabenazine-OHAAsamino acidsAMPHamphetaminebovine serum albuminBSACaCl(2)calcium chloridecounts per minuteCPMDATdecays per minutediolyl phosphatidyl serinedopamine transporterDPMeffluxglutathione-S-transferaseGSTHAhemagglutininkDaketansarinkeyhole limpet hemocyaninkilodaltonkinaseKLHKSRmagnesium chlorideMETHmethamphetamineMgCl(2)monoaminemonoamine plasma membrane transportersM-PMTsParkinson’s diseasePBSPDphosphate-buffered salinephosphatidylinositol-4,5-bisphosphatephospho-specific N-term antibodyPIP(2)PKCpN-term Abpolyvinylidene fluorideprotein kinase CPSPVDFreserpineRSPSDS–PAGESHsodium dodecyl sulfate–polyacrylamide gelsucrose HEPESTBZTBZ-OHtetrabenazinetransportervesicular monoamine transporter 2VMAT2

Identifiers

PMID24321511
PMCPMC3926813
OpenAlexW1984187378

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.