ArticleVirus research2014
Molecular modeling and analysis of hepatitis E virus (HEV) papain-like cysteine protease.
Article in Virus research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 29 citations in OpenAlex.
- Thrombin cleavage of the hepatitis E virus polyprotein at multiple conserved locations is required for genome replication.PLoS pathogens · 2023Article
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- Structural aspects of hepatitis E virus.Archives of virology · 2022Review
- Biochemical and Biophysical Characterisation of the Hepatitis E Virus Guanine-7-Methyltransferase.Molecules (Basel, Switzerland) · 2022Article
- Hepatitis E genotype 3 genome: A comprehensive analysis of entropy, motif conservation, relevant mutations, and clade-associated polymorphisms.Frontiers in microbiology · 2022Article
- Hepatitis E Virus Infection: Circulation, Molecular Epidemiology, and Impact on Global Health.Pathogens (Basel, Switzerland) · 2020Review
- Development of BacMam Induced Hepatitis E Virus Replication Model in Hepatoma Cells to Study the Polyprotein Processing.Frontiers in microbiology · 2020Article
- Hepatitis E Virus Replication.Viruses · 2019Review
- Biguanide is a modifiable pharmacophore for recruitment of endogenous ZnBiometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2019Review
- First Crystal Structure of a Nonstructural Hepatitis E Viral Protein Identifies a Putative Novel Zinc-Binding Protein.Journal of virology · 2019Article
- Hepatitis E Virus Cysteine Protease Has Papain Like Properties Validated byFrontiers in cellular and infection microbiology · 2019Article
- Analysis of Hepatitis E virus (HEV) X-domain structural model.Bioinformation · 2018Article
- Ancient recombination events and the origins of hepatitis E virus.BMC evolutionary biology · 2016Article
- Hepatitis E Virus Mutations: Functional and Clinical Relevance.EBioMedicine · 2016Review
- Article
- The Hepatitis E virus intraviral interactome.Scientific reports · 2015Article
- The lysine residues within the human ribosomal protein S17 sequence naturally inserted into the viral nonstructural protein of a unique strain of hepatitis E virus are important for enhanced virus replication.Journal of virology · 2015Article
- Hepatitis E virus inhibits type I interferon induction by ORF1 products.Journal of virology · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The biochemical or biophysical characterization of a papain-like cysteine protease in HEV ORF1-encoded polyprotein still remains elusive. Very recently, we have demonstrated the indispensability of ORF1 protease-domain cysteines and histidines in HEV replication, ex vivo (Parvez, 2013). In this report, the polyprotein partial sequences of HEV strains and genetically-related RNA viruses were analyzed, in silico. Employing the consensus-prediction results of RUBV-p(150) protease as structural-template, a 3D model of HEV-protease was deduced. Similar to RUBV-p(150), a 'papain-like β-barrel fold' structurally confirmed the classification of HEV-protease. Further, we recognized a catalytic 'Cys434-His443' dyad homologue of RUBV-p(150) (Cys1152-His1273) and FMDV-L(pro) (Cys51-His148) in line with our previous mutational analysis that showed essentiality of 'His443' but not 'His590' in HEV viability. Moreover, a RUBV 'Zn(2+) binding motif' (Cys1167-Cys1175-Cys1178-Cys1225-Cys1227) equivalent of HEV was identified as 'Cys457-His458-Cys459 and Cys481-Cys483' residues within the 'β-barrel fold'. Notably, unlike RUBV, 'His458' also clustered therein, that was in conformity with the consensus cysteine protease 'Zn(2+)-binding motif'. By homology, we also proposed an overlapping 'Ca(2+)-binding site' 'D-X-[DNS]-[ILVFYW]-[DEN]-G-[GP]-XX-DE' signature, and a 'proline-rich motif' interacting 'tryptophan (W437-W472)' module in the modeled structure. Our analysis of the predicted model therefore, warrants critical roles of the 'catalytic dyad' and 'divalent metal-binding motifs' in HEV protease structural-integrity, ORF1 self-processing, and RNA replication. This however, needs further experimental validations.
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