ArticleTissue engineering. Part A2014
Chondrocyte culture in three dimensional alginate sulfate hydrogels promotes proliferation while maintaining expression of chondrogenic markers.
Article in Tissue engineering. Part A, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 111 citations in OpenAlex.
- The Effect of Physical, Biochemical, and Electrical Culture Conditions on Articular Chondrocytes Used for Cartilage Tissue Engineering: A Narrative Review.International journal of molecular sciences · 2026Review
- The Floor-Ceiling-Chip, or 2 × 2D = Pseudo-3D-Approaching 3D Cell Morphology and Organization between Two Opposing 2D Substrates with Cell-Adhesive Protein Micropatterns.Advanced healthcare materials · 2026Article
- Macroporous Alginate-PEG Hybrid Double Network Cryogels: Tuning Mechanics, Porosity, and Long-Term Growth Factor Release via Polymer Concentration, Ice Nucleation, and Sulfation.ACS applied bio materials · 2026Article
- Donor Variability and 3D Culture Models Influence Human Mesenchymal Stem Cell Differentiation.Tissue engineering. Part A · 2026Article
- Synthesis and Characterization of Glycosaminoglycan Mimetic Variants to Promote Chondrogenesis.ACS omega · 2025Article
- Characterization of Composite Agarose-Collagen Hydrogels for Chondrocyte Culture.Annals of biomedical engineering · 2025Article
- Maintaining the Cartilage Phenotype of Late-Passage Chondrocytes Using Salidroside, TGF-β, and Sulfated Alginate for Cartilage Tissue Engineering Applications.International journal of molecular sciences · 2024Article
- Regenerative Variability of Human Juvenile Chondrocyte Sheets From Different Cell Donors in an Athymic Rat Knee Chondral Defect Model.Cartilage · 2024Article
- Betulin Stimulates Osteogenic Differentiation of Human Osteoblasts-Loaded Alginate-Gelatin Microbeads.Bioengineering (Basel, Switzerland) · 2024Article
- Sulfated Hydrogels as Primary Intervertebral Disc Cell Culture Systems.Gels (Basel, Switzerland) · 2024Article
- Human Chondrocytes, Metabolism of Articular Cartilage, and Strategies for Application to Tissue Engineering.International journal of molecular sciences · 2023Review
- 3D-Cultured Adipose-Derived Stem Cell Spheres Using Calcium-Alginate Scaffolds for Osteoarthritis Treatment in a Mono-Iodoacetate-Induced Rat Model.International journal of molecular sciences · 2023Article
- Dynamic Alginate Hydrogel as an Antioxidative Bioink for Bioprinting.Gels (Basel, Switzerland) · 2023Article
- Self-Healing Injectable Hydrogels for Tissue Regeneration.Chemical reviews · 2023Review
- Exploration of Dual Ionic Cross-Linked Alginate Hydrogels Via Cations of Varying Valences towards Wound Healing.Polymers · 2022Article
- Human Lung Organoid Culture in Alginate With and Without Matrigel to Model Development and Disease.Tissue engineering. Part A · 2022Article
- Stability of Biomimetically Functionalised Alginate Microspheres as 3D Support in Cell Cultures.Polymers · 2022Article
- The Endplate Role in Degenerative Disc Disease Research: The Isolation of Human Chondrocytes from Vertebral Endplate-An Optimised Protocol.Bioengineering (Basel, Switzerland) · 2022Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The loss of expression of chondrogenic markers during monolayer expansion remains a stumbling block for cell-based treatment of cartilage lesions. Here, we introduce sulfated alginate hydrogels as a cartilage biomimetic biomaterial that induces cell proliferation while maintaining the chondrogenic phenotype of encapsulated chondrocytes. Hydroxyl groups of alginate were converted to sulfates by incubation with sulfur trioxide-pyridine complex (SO3/pyridine), yielding a sulfated material cross-linkable with calcium chloride. Passage 3 bovine chondrocytes were encapsulated in alginate and alginate sulfate hydrogels for up to 35 days. Cell proliferation was five-fold higher in alginate sulfate compared with alginate (p=0.038). Blocking beta1 integrins in chondrocytes within alginate sulfate hydrogels significantly inhibited proliferation (p=0.002). Sulfated alginate increased the RhoA activity of chondrocytes compared with unmodified alginate, an increase that was blocked by β1 blocking antibodies (p=0.017). Expression and synthesis of type II collagen, type I collagen, and proteoglycan was not significantly affected by the encapsulation material evidenced by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunohistochemistry. Alginate sulfate constructs showed an opaque appearance in culture, whereas the unmodified alginate samples remained translucent. In conclusion, alginate sulfate provides a three dimensional microenvironment that promotes both chondrocyte proliferation and maintenance of the chondrogenic phenotype and represents an important advance for chondrocyte-based cartilage repair therapies providing a material in which cell expansion can be done in situ.
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