ArticleBioanalysis2013
Human platelets as a platform to monitor metabolic biomarkers using stable isotopes and LC-MS.
Article in Bioanalysis, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
- Safety, pharmacodynamics, and potential benefit of omaveloxolone in Friedreich ataxia.Annals of clinical and translational neurology · 2019Trial
- Role of frataxin protein deficiency and metabolic dysfunction in Friedreich ataxia, an autosomal recessive mitochondrial disease.Neuronal signaling · 2018Review
- Liquid Chromatography-High Resolution Mass Spectrometry Analysis of Platelet Frataxin as a Protein Biomarker for the Rare Disease Friedreich's Ataxia.Analytical chemistry · 2018Article
- Metabolic tracing analysis reveals substrate-specific metabolic deficits in platelet storage lesion.Transfusion · 2017Article
- Company profile: BluePen Biomarkers LLC – integrated biomarker solutions.Future science OA · 2016Article
- LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism.Journal of visualized experiments : JoVE · 2016Article
- Frataxin levels in peripheral tissue in Friedreich ataxia.Annals of clinical and translational neurology · 2015Article
- Metabolism of propionic acid to a novel acyl-coenzyme A thioester by mammalian cell lines and platelets.Journal of lipid research · 2015Article
- Translational metabolomics in cancer research.Biomarkers in medicine · 2015Review
- Stable isotopes and LC-MS for monitoring metabolic disturbances in Friedreich's ataxia platelets.Bioanalysis · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundIntracellular metabolites such as CoA thioesters are modulated in a number of clinical settings. Their accurate measurement from surrogate tissues such as platelets may provide additional information to current serum and urinary biomarkers.
methodsFreshly isolated platelets from healthy volunteers were treated with rotenone, propionate or isotopically labeled metabolic tracers. Using a recently developed LC-MS-based methodology, absolute changes in short-chain acyl-CoA thioesters were monitored, as well as relative metabolic labeling using isotopomer distribution analysis.
resultsConsistent with in vitro experiments, isolated platelets treated with rotenone showed decreased intracellular succinyl-CoA and increased β-hydroxybutyryl-CoA, while propionate treatment resulted in increased propionyl-CoA. In addition, isotopomers of the CoAs were readily detected in platelets treated with the [(13)C]- or [(13)C(15)N]-labeled metabolic precursors.
conclusionHere, we show that human platelets can provide a powerful ex vivo challenge platform with potential clinical diagnostic and biomarker discovery applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.