ArticleStem cell reports2013
Robust self-renewal of rat embryonic stem cells requires fine-tuning of glycogen synthase kinase-3 inhibition.
Article in Stem cell reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed, 68 citations in OpenAlex.
- Article
- Xenophagocytosis blockade enhances interspecies chimerism.bioRxiv : the preprint server for biology · 2025Article
- Inhibition of PRC2 enables self-renewal of blastoid-competent naive pluripotent stem cells from chimpanzee.Cell stem cell · 2025Article
- Capture primed pluripotency in guinea pig.Stem cell reports · 2025Article
- Relationship of PSC to embryos: Extending and refining capture of PSC lines from mammalian embryos.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024Review
- Propagating pluripotency - The conundrum of self-renewal.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024Review
- Hallmarks of totipotent and pluripotent stem cell states.Cell stem cell · 2024Review
- Harnessing the Stem Cell Niche in Regenerative Medicine: Innovative Avenue to Combat Neurodegenerative Diseases.International journal of molecular sciences · 2024Review
- Rat post-implantation epiblast-derived pluripotent stem cells produce functional germ cells.Cell reports methods · 2023Article
- Genome-scale screening in a rat haploid system identifies Thop1 as a modulator of pluripotency exit.Cell proliferation · 2022Article
- A Stem Cell Reporter for Investigating Pluripotency and Self-Renewal in the Rat.Stem cell reports · 2020Article
- PIXUL-ChIP: integrated high-throughput sample preparation and analytical platform for epigenetic studies.Nucleic acids research · 2019Article
- Establishment of porcine and human expanded potential stem cells.Nature cell biology · 2019Article
- Insulin signaling acts in adult adipocytes via GSK-3β and independently of FOXO to control Drosophila female germline stem cell numbers.Developmental biology · 2018Article
- Article
- A Chemical-Genetic Approach Reveals the Distinct Roles of GSK3α and GSK3β in Regulating Embryonic Stem Cell Fate.Developmental cell · 2017Article
- Rat embryonic stem cells produce fertile offspring through tetraploid complementation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Gene Editing in Rat Embryonic Stem Cells to Produce In Vitro Models and In Vivo Reporters.Stem cell reports · 2017Article
- Capturing Human Naïve Pluripotency in the Embryo and in the Dish.Stem cells and development · 2017Review
- From engineering to editing the rat genome.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Germline-competent embryonic stem cells (ESCs) have been derived from mice and rats using culture conditions that include an inhibitor of glycogen synthase kinase 3 (GSK3). However, rat ESCs remain susceptible to sporadic differentiation. Here, we show that unsolicited differentiation is attributable to overinhibition of GSK3. The self-renewal effect of inhibiting GSK3 is mediated via β-catenin, which abrogates the repressive action of TCF3 on core pluripotency genes. In rat ESCs, however, GSK3 inhibition also leads to activation of differentiation-associated genes, notably lineage specification factors Cdx2 and T. Lowered GSK3 inhibition reduces differentiation and enhances clonogenicity and self-renewal. The differential sensitivity of rat ESCs to GSK3 inhibition is linked to elevated expression of the canonical Wnt pathway effector LEF1. These findings reveal that optimal GSK3 inhibition for ESC propagation is influenced by the balance of TCF/LEF factors and can vary between species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.