ArticlePloS one2013
Rapid mutation of Spirulina platensis by a new mutagenesis system of atmospheric and room temperature plasmas (ARTP) and generation of a mutant library with diverse phenotypes.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 128 citations in OpenAlex.
- Current breakthroughs and advances in atmospheric room temperature plasma (ARTP) technology for biomanufacturing.Bioresources and bioprocessing · 2025Review
- Impact of nutrient deficiency and harvesting strategy on biomass and phycocyanin production inFrontiers in bioengineering and biotechnology · 2025Article
- Selection and Genetic Analysis of High Polysaccharide-Producing Mutants inMicroorganisms · 2024Article
- Identification of the mutual gliding locus as a factor for gut colonization in non-native bee hosts using the ARTP mutagenesis.Microbiome · 2024Article
- Application of Atmospheric and Room-Temperature Plasma (ARTP) to Microbial Breeding.Current issues in molecular biology · 2023Review
- Metabolic analysis of Schizochytrium sp. mutants with high EPA content achieved with ARTP mutagenesis screening.Bioprocess and biosystems engineering · 2023Article
- Enhancing wuyiencin productivity of Streptomyces albulus (CK15) by mutagenesis breeding with atmospheric and room temperature plasma.World journal of microbiology & biotechnology · 2023Article
- Enhancing biomass and lipid productivity of a green microalga Parachlorella kessleri for biodiesel production using rapid mutation of atmospheric and room temperature plasma.Biotechnology for biofuels and bioproducts · 2022Article
- Strain Development in Microalgal Biotechnology-Random Mutagenesis Techniques.Life (Basel, Switzerland) · 2022Review
- Article
- ARTP and NTG compound mutations improved Cry protein production and virulence of Bacillus thuringiensis X023.Applied microbiology and biotechnology · 2022Article
- Improved Neomycin Sulfate Potency inMicroorganisms · 2022Article
- ARTP Mutagenesis ofMarine drugs · 2021Article
- Metabolic Engineering for Carotenoid Production Using Eukaryotic Microalgae and Prokaryotic Cyanobacteria.Advances in experimental medicine and biology · 2021Article
- From nature to nurture: Essence and methods to isolate robust methanotrophic bacteria.Synthetic and systems biotechnology · 2020Article
- The Structural Characteristics and Biological Activities of Intracellular Polysaccharide Derived from MutagenicMolecules (Basel, Switzerland) · 2020Article
- Combined mutagenesis and metabolic regulation to enhance D-arabitol production from Candida parapsilosis.Journal of industrial microbiology & biotechnology · 2020Article
- Screening of a highly inhibitor-tolerant bacterial strain for 2,3-BDO and organic acid production from non-detoxified corncob acid hydrolysate.AMB Express · 2019Article
- Novel breeding approach for Japanese flounder using atmosphere and room temperature plasma mutagenesis tool.BMC genomics · 2019Article
- Plasma-sensitive Escherichia coli mutants reveal plasma resistance mechanisms.Journal of the Royal Society, Interface · 2019Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this paper, we aimed to improve the carbohydrate productivity of Spirulina platensis by generating mutants with increased carbohydrate content and growth rate. ARTP was used as a new mutagenesis tool to generate a mutant library of S. platensis with diverse phenotypes. Protocol for rapid mutation of S. platensis by 60 s treatment with helium driven ARTP and high throughput screening method of the mutants using the 96-well microplate and microplate reader was established. A mutant library of 62 mutants was then constructed and ideal mutants were selected out. The characteristics of the mutants after the mutagenesis inclined to be stable after around 9(th) subculture, where the total mutation frequency and positive mutation frequency in terms of specific growth rate reached 45% and 25%, respectively. The mutants in mutant library showed diverse phenotypes in terms of cell growth rate, carbohydrate content and flocculation intensity. The positive mutation frequency in terms of cellular carbohydrate content with the increase by more than 20% percent than the wild strain was 32.3%. Compared with the wild strain, the representative mutants 3-A10 and 3-B2 showed 40.3% and 78.0% increase in carbohydrate content, respectively, while the mutant 4-B3 showed 10.5% increase in specific growth rate. The carbohydrate contents of the representative mutants were stable during different subcultures, indicating high genetic stability. ARTP was demonstrated to be an effective and non-GMO mutagenesis tool to generate the mutant library for multicellular microalgae.
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