ArticleJournal of immunology (Baltimore, Md. : 1950)2014
The pore-forming toxin listeriolysin O is degraded by neutrophil metalloproteinase-8 and fails to mediate Listeria monocytogenes intracellular survival in neutrophils.
Article in Journal of immunology (Baltimore, Md. : 1950), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Heparin-binding enhances extracellular listeriolysin O activity, overcoming cholesterol inhibition and pH dependence.Journal of bacteriology · 2026Article
- Liposomal Nanotraps NeutralizeInfectious microbes & diseases · 2025Article
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- Executioner caspases degrade essential mediators of pathogen-host interactions to inhibit growth of intracellular Listeria monocytogenes.Cell death & disease · 2025Article
- Integrated metagenomics and transcriptomics analysis reveals pathways associated with oral periapical lesions formation and progression.Current research in microbial sciences · 2025Article
- Genomic and pathogenicity islands ofFrontiers in molecular biosciences · 2023Review
- Myeloperoxidase: Regulation of Neutrophil Function and Target for Therapy.Antioxidants (Basel, Switzerland) · 2022Review
- Listeria exploits IFITM3 to suppress antibacterial activity in phagocytes.Nature communications · 2021Article
- Article
- Therapeutic targeting of neutrophil exocytosis.Journal of leukocyte biology · 2020Review
- Human Neutrophil Granule Exocytosis in Response toPathogens (Basel, Switzerland) · 2020Article
- Neutrophil pyroptosis: new perspectives on sepsis.Cellular and molecular life sciences : CMLS · 2019Review
- Innate and Adaptive Immune Responses duringMicrobiology spectrum · 2019Article
- Relative Roles of Listeriolysin O, InlA, and InlB in Listeria monocytogenes Uptake by Host Cells.Infection and immunity · 2018Article
- Neutrophils from Both Susceptible and Resistant Mice Efficiently Kill Opsonized Listeria monocytogenes.Infection and immunity · 2018Article
- Proteogenomics in Aid of Host-Pathogen Interaction Studies: A Bacterial Perspective.Proteomes · 2017Review
- Listeriolysin O: from bazooka to Swiss army knife.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Monocytes Are the Predominant Cell Type Associated withJournal of immunology (Baltimore, Md. : 1950) · 2017Article
- Control of Phagocytosis by Microbial Pathogens.Frontiers in immunology · 2017Review
- High-Throughput Microplate-Based Assay to Monitor Plasma Membrane Wounding and Repair.Frontiers in cellular and infection microbiology · 2017Article
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Authors and funding
8 authors.
Funding
Abstract
The pore-forming toxin listeriolysin O (LLO) is a major virulence factor secreted by the facultative intracellular pathogen Listeria monocytogenes. This toxin facilitates L. monocytogenes intracellular survival in macrophages and diverse nonphagocytic cells by disrupting the internalization vesicle, releasing the bacterium into its replicative niche, the cytosol. Neutrophils are innate immune cells that play an important role in the control of infections, yet it was unknown if LLO could confer a survival advantage to L. monocytogenes in neutrophils. We report that LLO can enhance the phagocytic efficiency of human neutrophils and is unable to protect L. monocytogenes from intracellular killing. To explain the absence of L. monocytogenes survival in neutrophils, we hypothesized that neutrophil degranulation leads to the release of LLO-neutralizing molecules in the forming phagosome. In support of this, L. monocytogenes is a potent inducer of neutrophil degranulation, since its virulence factors, such as LLO, facilitate granule exocytosis. Within the first few minutes of interaction with L. monocytogenes, granules can fuse with the plasma membrane at the bacterial interaction site before closure of the phagosome. Furthermore, granule products directly degrade LLO, irreversibly inhibiting its activity. The matrix metalloproteinase-8, stored in secondary granules, was identified as an endoprotease that degrades LLO, and blocking neutrophil proteases increased L. monocytogenes intracellular survival. In conclusion, we propose that LLO degradation by matrix metalloproteinase-8 during phagocytosis protects neutrophil membranes from perforation and contributes to maintaining L. monocytogenes in a bactericidal phagosome from which it cannot escape.
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