ArticleNucleic acids research2014
Identification of genes involved in low aminoglycoside-induced SOS response in Vibrio cholerae: a role for transcription stalling and Mfd helicase.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Genomic evolution and stability of the mcr-1-harboring IncI2 plasmid in the presence and absence of colistin.BMC genomics · 2025Article
- RNA polymerase stalling-derived genome instability underlies ribosomal antibiotic efficacy and resistance evolution.Nature communications · 2024Article
- RavA-ViaA antibiotic response is linked to Cpx and Zra2 envelope stress systems inMicrobiology spectrum · 2023Article
- Article
- Biochemical and Structural Analyses Shed Light on the Mechanisms of RadD DNA Binding and Its ATPase fromInternational journal of molecular sciences · 2023Article
- The AAA+ ATPase RavA and its binding partner ViaA modulate E. coli aminoglycoside sensitivity through interaction with the inner membrane.Nature communications · 2022Article
- AeLife · 2022Article
- Nonessential tRNA and rRNA modifications impact the bacterial response to sub-MIC antibiotic stress.microLife · 2022Article
- Link Between Antibiotic Persistence and Antibiotic Resistance in Bacterial Pathogens.Frontiers in cellular and infection microbiology · 2022Review
- Single-molecule studies of helicases and translocases in prokaryotic genome-maintenance pathways.DNA repair · 2021Review
- Interplay between Sublethal Aminoglycosides and Quorum Sensing: Consequences on Survival inCells · 2021Article
- Sleeping ribosomes: Bacterial signaling triggers RaiA mediated persistence to aminoglycosides.iScience · 2021Article
- Deficiency in cytosine DNA methylation leads to high chaperonin expression and tolerance to aminoglycosides in Vibrio cholerae.PLoS genetics · 2021Article
- Article
- Expansion of the SOS regulon of Vibrio cholerae through extensive transcriptome analysis and experimental validation.BMC genomics · 2018Article
- Escherichia coli RadD Protein Functionally Interacts with the Single-stranded DNA-binding Protein.The Journal of biological chemistry · 2016Article
- Dynamics of a Class 1 Integron Located on Plasmid or Chromosome in TwoFrontiers in microbiology · 2016Article
- Escherichia coli radD (yejH) gene: a novel function involved in radiation resistance and double-strand break repair.Molecular microbiology · 2015Article
- Biological cost of pyocin production during the SOS response in Pseudomonas aeruginosa.Journal of bacteriology · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sub-inhibitory concentrations (sub-MIC) of antibiotics play a very important role in selection and development of resistances. Unlike Escherichia coli, Vibrio cholerae induces its SOS response in presence of sub-MIC aminoglycosides. A role for oxidized guanine residues was observed, but the mechanisms of this induction remained unclear. To select for V. cholerae mutants that do not induce low aminoglycoside-mediated SOS induction, we developed a genetic screen that renders induction of SOS lethal. We identified genes involved in this pathway using two strategies, inactivation by transposition and gene overexpression. Interestingly, we obtained mutants inactivated for the expression of proteins known to destabilize the RNA polymerase complex. Reconstruction of the corresponding mutants confirmed their specific involvement in induction of SOS by low aminoglycoside concentrations. We propose that DNA lesions formed on aminoglycoside treatment are repaired through the formation of single-stranded DNA intermediates, inducing SOS. Inactivation of functions that dislodge RNA polymerase leads to prolonged stalling on these lesions, which hampers SOS induction and repair and reduces viability under antibiotic stress. The importance of these mechanisms is illustrated by a reduction of aminoglycoside sub-MIC. Our results point to a central role for transcription blocking at DNA lesions in SOS induction, so far underestimated.
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