ArticleNucleic acids research2014
A cypovirus VP5 displays the RNA chaperone-like activity that destabilizes RNA helices and accelerates strand annealing.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- Mutation in enterovirus 71 nonstructural protein 3A increases genome replication fidelity and exhibits attenuated virulence in mice.Journal of virology · 2025Article
- Respiratory syncytial virus phosphoprotein has NTPase and helicase-like activities.Journal of virology · 2025Article
- Structure of orthoreovirus RNA chaperone σNS, a component of viral replication factories.Nature communications · 2024Article
- Integration of Cypoviruses into polyhedrin matrix.Nanoscale advances · 2023Article
- Heterogeneous Nuclear Protein U Degraded the mJournal of virology · 2023Article
- SARS-CoV-2 helicase NSP13 hijacks the host protein EWSR1 to promote viral replication by enhancing RNA unwinding activity.Infectious medicine · 2022Article
- ATPase and helicase activities of porcine epidemic diarrhea virus nsp13.Veterinary microbiology · 2021Article
- Antiviral Peptides Targeting the Helicase Activity of Enterovirus Nonstructural Protein 2C.Journal of virology · 2021Article
- SARS-Coronavirus-2 Nsp13 Possesses NTPase and RNA Helicase Activities That Can Be Inhibited by Bismuth Salts.Virologica Sinica · 2020Article
- Ebola virus VP35 has novel NTPase and helicase-like activities.Nucleic acids research · 2019Article
- Human Norovirus NS3 Has RNA Helicase and Chaperoning Activities.Journal of virology · 2018Article
- Cypovirus capsid protein VP5 has nucleoside triphosphatase activity.Virologica Sinica · 2017Article
- MTase Domain of Dendrolimus punctatus cypovirus VP3 Mediates Virion Attachment and Interacts with Host ALP Protein.Viruses · 2017Article
- RNA chaperones encoded by RNA viruses.Virologica Sinica · 2015Review
- Viral Small-RNA Analysis of Bombyx mori Larval Midgut during Persistent and Pathogenic Cytoplasmic Polyhedrosis Virus Infection.Journal of virology · 2015Article
- Human Enterovirus Nonstructural Protein 2CATPase Functions as Both an RNA Helicase and ATP-Independent RNA Chaperone.PLoS pathogens · 2015Article
- The identification and characterization of nucleic acid chaperone activity of human enterovirus 71 nonstructural protein 3AB.Virology · 2014Article
- Genomic and biological characterization of a new cypovirus isolated from Dendrolimus punctatus.PloS one · 2014Article
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For double-stranded RNA (dsRNA) viruses in the family Reoviridae, their inner capsids function as the machinery for viral RNA (vRNA) replication. Unlike other multishelled reoviruses, cypovirus has a single-layered capsid, thereby representing a simplified model for studying vRNA replication of reoviruses. VP5 is one of the three major cypovirus capsid proteins and functions as a clamp protein to stabilize cypovirus capsid. Here, we expressed VP5 from type 5 Helicoverpa armigera cypovirus (HaCPV-5) in a eukaryotic system and determined that this VP5 possesses RNA chaperone-like activity, which destabilizes RNA helices and accelerates strand annealing independent of ATP. Our further characterization of VP5 revealed that its helix-destabilizing activity is RNA specific, lacks directionality and could be inhibited by divalent ions, such as Mg(2+), Mn(2+), Ca(2+) or Zn(2+), to varying degrees. Furthermore, we found that HaCPV-5 VP5 facilitates the replication initiation of an alternative polymerase (i.e. reverse transcriptase) through a panhandle-structured RNA template, which mimics the 5'-3' cyclization of cypoviral positive-stranded RNA. Given that the replication of negative-stranded vRNA on the positive-stranded vRNA template necessitates the dissociation of the 5'-3' panhandle, the RNA chaperone activity of VP5 may play a direct role in the initiation of reoviral dsRNA synthesis.
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