ArticleMolecular & cellular proteomics : MCP2014
Correlation of actin crosslinker and capper expression levels with stereocilia growth phases.
Article in Molecular & cellular proteomics : MCP, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Rescue of stereocilia architecture and hearing function by AAV-CIB2 and AAV-CIB3 in a mouse model of recessive deafness DFNB48.Molecular therapy : the journal of the American Society of Gene Therapy · 2026Article
- Inhibition of the ILK-AKT pathway by upregulation of PARVB contributes to the cochlear cell death in Fascin2 gene knockout mice.Cell death discovery · 2024Article
- Control of stereocilia length during development of hair bundles.PLoS biology · 2023Article
- Actin Bundles Dynamics and Architecture.Biomolecules · 2023Review
- A combined genome-wide association and molecular study of age-related hearing loss in H. sapiens.BMC medicine · 2021Article
- Article
- Building and repairing the stereocilia cytoskeleton in mammalian auditory hair cells.Hearing research · 2019Review
- Molecular Composition of Vestibular Hair Bundles.Cold Spring Harbor perspectives in medicine · 2019Review
- Null Mutation of theG3 (Bethesda, Md.) · 2018Article
- Article
- Heterodimeric capping protein is required for stereocilia length and width regulation.The Journal of cell biology · 2017Article
- Stereocilia morphogenesis and maintenance through regulation of actin stability.Seminars in cell & developmental biology · 2017Review
- Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid.The Journal of cell biology · 2016Article
- Article
- Article
- Assembly of hair bundles, an amazing problem for cell biology.Molecular biology of the cell · 2015Review
- Article
- Genetics of auditory mechano-electrical transduction.Pflugers Archiv : European journal of physiology · 2015Review
- The proteome of mouse vestibular hair bundles over development.Scientific data · 2015Article
- Quantitative trait loci on chromosome 5 for susceptibility to frequency-specific effects on hearing in DBA/2J mice.Experimental animals · 2015Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
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Abstract
During development of the chick cochlea, actin crosslinkers and barbed-end cappers presumably influence growth and remodeling of the actin paracrystal of hair cell stereocilia. We used mass spectrometry to identify and quantify major actin-associated proteins of the cochlear sensory epithelium from E14 to E21, when stereocilia widen and lengthen. Tight actin crosslinkers (i.e. fascins, plastins, and espin) are expressed dynamically during cochlear epithelium development between E7 and E21, with FSCN2 replacing FSCN1 and plastins remaining low in abundance. Capping protein, a barbed-end actin capper, is located at stereocilia tips; it is abundant during growth phase II, when stereocilia have ceased elongating and are increasing in diameter. Capping protein levels then decline during growth phase III, when stereocilia reinitiate barbed-end elongation. Although actin crosslinkers are readily detected by electron microscopy in developing chick cochlea stereocilia, quantitative mass spectrometry of stereocilia isolated from E21 chick cochlea indicated that tight crosslinkers are present there in stoichiometric ratios relative to actin that are much lower than their ratios for vestibular stereocilia. These results demonstrate the value of quantitation of global protein expression in chick cochlea during stereocilia development.
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