ArticlePhotosynthesis research1993
Photoproduction of hydrogen peroxide in Photosystem II membrane fragments: A comparison of four signals.
Article in Photosynthesis research, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 76 citations in OpenAlex.
- Oxygen and ROS in Photosynthesis.Plants (Basel, Switzerland) · 2020Review
- Hydrogen Peroxide and Superoxide Anion Radical Photoproduction in PSII Preparations at Various Modifications of the Water-Oxidizing Complex.Plants (Basel, Switzerland) · 2019Article
- Hydroxyectoine protects Mn-depleted photosystem II against photoinhibition acting as a source of electrons.Photosynthesis research · 2019Article
- Thomas John Wydrzynski (8 July 1947-16 March 2018).Photosynthesis research · 2019Article
- Oxygenic Photoreactivity in Photosystem II Studied by Rotating Ring Disk Electrochemistry.Journal of the American Chemical Society · 2018Article
- Vyacheslav (Slava) Klimov (1945-2017): A scientist par excellence, a great human being, a friend, and a Renaissance man.Photosynthesis research · 2018Article
- Two pathways of photoproduction of organic hydroperoxides on the donor side of photosystem 2 in subchloroplast membrane fragments.Photosynthesis research · 2017Article
- Gernot Renger (1937-2013): his life, Max-Volmer Laboratory, and photosynthesis research.Photosynthesis research · 2016Article
- Origin and Evolution of Water Oxidation before the Last Common Ancestor of the Cyanobacteria.Molecular biology and evolution · 2015Article
- Flash-induced consumption of molecular oxygen on the donor side of photosystem II in Mn-depleted subchloroplast membrane fragments: specific effects of manganese and calcium ions.Photosynthesis research · 2013Article
- Quality control of photosystem II: lipid peroxidation accelerates photoinhibition under excessive illumination.PloS one · 2012Article
- Damage to the oxygen-evolving complex by superoxide anion, hydrogen peroxide, and hydroxyl radical in photoinhibition of photosystem II.Photosynthesis research · 2006Article
- PsbP protein, but not PsbQ protein, is essential for the regulation and stabilization of photosystem II in higher plants.Plant physiology · 2005Article
- Superoxide, Hydrogen Peroxide and Hydroxyl Radical in D1/D2/cytochrome b-559 Photosystem II Reaction Center Complex.Photosynthesis research · 2004Article
- Stepwise transition of the tetra-manganese complex of photosystem II to a binuclear Mn2(micro -O)2 complex in response to a temperature jump: a time-resolved structural investigation employing x-ray absorption spectroscopy.Biophysical journal · 2003Article
- Oxygen reduction in a plastoquinone pool of isolated pea thylakoids.Photosynthesis research · 2002Article
- The structure and function of CP47 and CP43 in Photosystem II.Photosynthesis research · 2002Article
- Binary oscillations in the Kok model of oxygen evolution in oxygenic photosynthesis.Photosynthesis research · 1996Article
- Inactivation of the water-oxidizing enzyme in manganese stabilizing protein-free mutant cells of the cyanobacteria Synechococcus PCC7942 and Synechocystic PCC6803 during dark incubation and conditions leading to photoactivation.Photosynthesis research · 1994Article
- The photoproduction of superoxide radicals and the superoxide dismutase activity of Photosystem II. The possible involvement of cytochrome b559.Photosynthesis research · 1994Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study describes the formation of different forms of peroxide in Photosystem II (PS II) by using a chemiluminescence detection technique. Four chemiluminescence signals (A, B, C and D) of the luminolperoxidase (Lu-Per) system, which detects peroxide, are found in illuminated O2-evolving Photosystem II (PS II) membrane fragments isolated from spinach. Signal A ('free peroxide') peaking around 0.2-0.3 s after mixing PS II membrane fragments with Lu-Per is eliminated by catalase or removal of oxygen from the suspension and ascribed to O2 interaction with reduced PS II electron acceptors. In contrast, signal B peaking around 1.5 min remains largely unaffected under anaerobic conditions, as well as in the presence of catalase (20 μg/ml). Under flash illumination the extent of this signal exhibits a weak period four oscillation (maximum at third and 7th flash). Its yield increases up to the third flash, but is close to zero in the fourth flash. An analogous behaviour is observed in flashes 5 to 8. Signal B is ascribed to Lu-Per interaction with the water-oxidizing system being in S2 and/or S3-state. Signal C ('bound peroxide') detected as free peroxide after acid decomposition of illuminated PS II particles is observed on the 1 st flash and oscillates with period 2 with superposition of period 4. It is evidently related to peroxide either released from S2 or formed at S2 upon acid shock treatment. Signal D ('slowly released peroxide') peaking around 2-3 s after mixing is observed in samples after various treatments (LCC-incubation, washing with 1 M NaCl at pH 8 or with 1 M CaCl2, Cl(-)-depletion) that lead to at least partial removal of the extrinsic proteins of 18, 24 and 33 kDa without Mn extraction. The average amplitude of this signal corresponds with a yield of about 0.2 H2O2 molecules per RC and flash. In a flash train, the extent of signal D exhibits an oscillation pattern with a minimum at the 3rd flash. We assume that these treatments increase the release of 'bound' peroxide (upon injection into the Lu-Per assay) either formed in the normal oxidative pathway of the water oxidase in the S2 or the S3-state or give rise to peroxide formation due to higher accessibility of the Mn-cluster to water molecules.
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