ArticleInternational journal of molecular sciences2013
Mutation in the pssA gene involved in exopolysaccharide synthesis leads to several physiological and symbiotic defects in Rhizobium leguminosarum bv. trifolii.
Article in International journal of molecular sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 26 citations in OpenAlex.
- Roles of plasmid-borneFrontiers in plant science · 2026Article
- Exopolysaccharide Biosynthesis inInternational journal of molecular sciences · 2023Article
- A New Face of the Old Gene: Deletion of theInternational journal of molecular sciences · 2023Article
- The Ros/MucR Zinc-Finger Protein Family in Bacteria: Structure and Functions.International journal of molecular sciences · 2022Review
- Rhizobial Exopolysaccharides: Genetic Regulation of Their Synthesis and Relevance in Symbiosis with Legumes.International journal of molecular sciences · 2021Review
- 1-Aminocyclopropane-1-Carboxylate Deaminase-Producing Plant Growth-Promoting Rhizobacteria Improve Drought Stress Tolerance in Grapevine (Frontiers in plant science · 2021Article
- PssJ Is a Terminal Galactosyltransferase Involved in the Assembly of the Exopolysaccharide Subunit inInternational journal of molecular sciences · 2020Article
- Transcriptomic Studies Reveal that theInternational journal of molecular sciences · 2019Article
- Mutation in theGenes · 2018Article
- Rhizobia: from saprophytes to endosymbionts.Nature reviews. Microbiology · 2018Review
- Synthesis of Rhizobial Exopolysaccharides and Their Importance for Symbiosis with Legume Plants.Genes · 2017Review
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- Symbiotic nitrogen fixation in legume nodules: metabolism and regulatory mechanisms.International journal of molecular sciences · 2014Article
- Exopolysaccharide biosynthesis enables mature biofilm formation on abiotic surfaces by Herbaspirillum seropedicae.PloS one · 2014Article
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2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The symbiotic nitrogen-fixing bacterium Rhizobium leguminosarum bv. trifolii 24.2 secretes large amounts of acidic exopolysaccharide (EPS), which plays a crucial role in establishment of effective symbiosis with clover. The biosynthesis of this heteropolymer is conducted by a multi-enzymatic complex located in the bacterial inner membrane. PssA protein, responsible for the addition of glucose-1-phosphate to a polyprenyl phosphate carrier, is involved in the first step of EPS synthesis. In this work, we characterize R. leguminosarum bv. trifolii strain Rt270 containing a mini-Tn5 transposon insertion located in the 3'-end of the pssA gene. It has been established that a mutation in this gene causes a pleiotropic effect in rhizobial cells. This is confirmed by the phenotype of the mutant strain Rt270, which exhibits several physiological and symbiotic defects such as a deficiency in EPS synthesis, decreased motility and utilization of some nutrients, decreased sensitivity to several antibiotics, an altered extracellular protein profile, and failed host plant infection. The data of this study indicate that the protein product of the pssA gene is not only involved in EPS synthesis, but also required for proper functioning of Rhizobium leguminosarum bv. trifolii cells.
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