ArticleNature methods2014
Quantifying cell-generated mechanical forces within living embryonic tissues.
Article in Nature methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 225 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
225 citing papers in PubMed, 469 citations in OpenAlex.
- Mechanical stress remodelling drives epithelial-mesenchymal transition in the tumour microenvironment.Nature nanotechnology · 2026Article
- Harnessing microfluidics for microbiology: from bacteria-host interactions to emerging cancer therapies.Communications biology · 2026Review
- Decoding mechanoregulation in immunological synapses using biomimetic artificial cells.Nature methods · 2026Article
- Shape-dependent initial pressure effects of fluid inclusions in an elastic solid under plane deformation.Mathematics and mechanics of solids : MMS · 2026Article
- Mechanical control in dental and jaw morphogenesis and remodeling.International journal of oral science · 2026Review
- Mechanics of compression-driven morphogenesis.Development (Cambridge, England) · 2026Review
- TiFM2.0 - versatile mechanical measurement and actuation in live embryos.Development (Cambridge, England) · 2026Article
- Noninvasive 3D mapping of tissue stress using reverberant shear wave.Nature communications · 2026Article
- Volume and surface methods for microparticle traction force microscopy: a computational and experimental comparison.Soft matter · 2026Article
- Decoding the origins of cellular self-organization for engineered biology.Nature biotechnology · 2026Review
- Necking of epithelial tissues with cellular topological transition.PLoS computational biology · 2026Article
- Counterion-Enhanced Brightness of Fluorous-Soluble Heptamethine Cyanine Dyes for Near- and Shortwave Infrared Fluorescence Imaging.Chemical & biomedical imaging · 2026Article
- Using tunable hydrogel microparticles to measure cellular forces.Nature protocols · 2026Review
- Stress-dependent growth in breast cancer arises from a mechano-osmotic coupling and cell-sizing checkpoint.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Scale-Specific Viscoelastic Characterization of Hydrogels: Integrated AFM and Finite Element Modeling.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- MultiCell: geometric learning in multicellular development.Nature methods · 2026Article
- Unified Transcriptome and Mechanics Map of the Intact Mammalian Preimplantation Embryo In Situ.bioRxiv : the preprint server for biology · 2026Article
- Design and optimization of in situ self-functionalizing stress sensors.The European physical journal. E, Soft matter · 2026Article
- 3D biomimetic niche modulates embryo development in vitro.Nature communications · 2026Article
- Mechanical constraints on cell plasticity: insights from the olfactory epithelium.Frontiers in cell and developmental biology · 2026Review
165 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Cell-generated mechanical forces play a critical role during tissue morphogenesis and organ formation in the embryo. Little is known about how these forces shape embryonic organs, mainly because it has not been possible to measure cellular forces within developing three-dimensional (3D) tissues in vivo. We present a method to quantify cell-generated mechanical stresses exerted locally within living embryonic tissues, using fluorescent, cell-sized oil microdroplets with defined mechanical properties and coated with adhesion receptor ligands. After a droplet is introduced between cells in a tissue, local stresses are determined from droplet shape deformations, measured using fluorescence microscopy and computerized image analysis. Using this method, we quantified the anisotropic stresses generated by mammary epithelial cells cultured within 3D aggregates, and we confirmed that these stresses (3.4 nN μm(-2)) are dependent on myosin II activity and are more than twofold larger than stresses generated by cells of embryonic tooth mesenchyme, either within cultured aggregates or in developing whole mouse mandibles.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.