ArticleApplied and environmental microbiology2014
Strain design of Ashbya gossypii for single-cell oil production.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 35 citations in OpenAlex.
- Lipid packing and local geometry influence septin curvature sensing.The Journal of cell biology · 2026Article
- Biosynthesis of Fatty Acid Derivatives by RecombinantJournal of fungi (Basel, Switzerland) · 2023Article
- New Promoters for Metabolic Engineering ofJournal of fungi (Basel, Switzerland) · 2021Article
- The Potential of Single-Cell Oils Derived From Filamentous Fungi as Alternative Feedstock Sources for Biodiesel Production.Frontiers in microbiology · 2021Review
- Metabolic engineering of Ashbya gossypii for deciphering the de novo biosynthesis of γ-lactones.Microbial cell factories · 2019Article
- Bioproduction of riboflavin: a bright yellow history.Journal of industrial microbiology & biotechnology · 2017Review
- Engineering Ashbya gossypii strains for de novo lipid production using industrial by-products.Microbial biotechnology · 2017Article
- Utilization of xylose by engineered strains ofBiotechnology for biofuels · 2017Article
- Guanine nucleotide binding to the Bateman domain mediates the allosteric inhibition of eukaryotic IMP dehydrogenases.Nature communications · 2015Article
- Metabolic engineering of riboflavin production in Ashbya gossypii through pathway optimization.Microbial cell factories · 2015Article
- Increased production of inosine and guanosine by means of metabolic engineering of the purine pathway in Ashbya gossypii.Microbial cell factories · 2015Article
- Engineering Ashbya gossypii for efficient biolipid production.Bioengineered · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-cell oil (SCO) represents a sustainable alternative for the oil industry. Accordingly, the identification of microorganisms with either higher lipidogenic ability or novel capacities for the transformation of raw materials constitutes a major challenge for the field of oil biotechnology. With this in mind, here, we were prompted to address the lipidogenic profile of the filamentous hemiascomycete Ashbya gossypii, which is currently used for the microbial production of vitamins. We found that A. gossypii mostly accumulates unsaturated fatty acids (FAs), with more than 50% of the total FA content corresponding to oleic acid. In addition, we engineered A. gossypii strains both lacking the beta-oxidation pathway and also providing ATP-citrate lyase (ACL) activity to block the degradation of FA and to increase the cytosolic acetyl-coenzyme A (CoA) content, respectively. The lipidogenic profile of the newly developed strains demonstrates that the mere elimination of the beta-oxidation pathway in A. gossypii triggers a significant increase in lipid accumulation that can reach 70% of cell dry weight. The use of A. gossypii as a novel and robust tool for the production of added-value oils is further discussed.
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