Evidence map›Paper›PMID 24316849›Full record

ArticleActa crystallographica. Section F, Structural biology and crystallization communications2013

Overexpression, crystallization and preliminary X-ray crystallographic analysis of glucuronoxylan xylanohydrolase (Xyn30A) from Clostridium thermocellum.

Anil Kumar Verma, Arun Goyal, Filipe Freire, Pedro Bule, Immacolata Venditto, Joana L A Brás, Helena Santos, Vânia Cardoso, Cecília Bonifácio, Andrew Thompson and 5 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta crystallographica. Section F, Structural biology and crystallization communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 4 institutions in 3 countries.

Anil Kumar VermaDepartment of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781 039, India.
Arun Goyal
Filipe Freire
Pedro Bule
Immacolata Venditto
Joana L A Brás
Helena Santos
Vânia Cardoso
Cecília Bonifácio
Andrew Thompson
Maria João Romão
José A M Prates
Luís M A Ferreira
Carlos M G A Fontes
Shabir Najmudin
Indian Institute of Technology Guwahati · INRede de Química e Tecnologia · PTSynchrotron soleil · FRUniversidade Nova de Lisboa · PT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The modular carbohydrate-active enzyme belonging to glycoside hydrolase family 30 (GH30) from Clostridium thermocellum (CtXynGH30) is a cellulosomal protein which plays an important role in plant cell-wall degradation. The full-length CtXynGH30 contains an N-terminal catalytic module (Xyn30A) followed by a family 6 carbohydrate-binding module (CBM6) and a dockerin at the C-terminus. The recombinant protein has a molecular mass of 45 kDa. Preliminary structural characterization was carried out on Xyn30A crystallized in different conditions. All tested crystals belonged to space group P1 with one molecule in the asymmetric unit. Molecular replacement has been used to solve the Xyn30A structure.

Indexed as

Acetivibrio thermocellusAmino Acid SequenceBacterial ProteinsCrystallizationCrystallography, X-RayEscherichia coliGene ExpressionHistidineMolecular Sequence DataOligopeptidesProtein Structure, TertiaryRecombinant Fusion ProteinsXylosidasesBacterial Proteinsglucuronoxylan xylanohydrolaseHis-His-His-His-His-HisHistidineOligopeptidesRecombinant Fusion ProteinsXylosidasesClostridium thermocellumglycoside hydrolase family 30Xyn30A

Identifiers

PMID24316849
PMCPMC3855739
OpenAlexW2326667268

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.