Evidence map›Paper›PMID 24316420›Full record

ArticleBone2014

Deletion of Mecom in mouse results in early-onset spinal deformity and osteopenia.

Subhash C Juneja, Alin Vonica, Caroline Zeiss, Kimberly Lezon-Geyda, Bogdan Yatsula, David R Sell, Vincent M Monnier, Sharon Lin, Thomas Ardito, David Eyre and 10 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bone, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
0.9field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it, 19 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Mds1Cell reports · 2021
    Article
  4. Article
  5. Review
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 4 institutions in 1 country.

Subhash C JunejaDepartment of Pathology and Laboratory Medicine, University of Rochester Medical Center, USA; Department of Orthopedics, University of Rochester Medical Center, USA; Department of Biomedical Engineering, University of Rochester Medical Center, USA. Electronic address: sjuneja.phd@gmail.com.
Alin VonicaDepartment of Pathology and Laboratory Medicine, University of Rochester Medical Center, USA. Electronic address: alin_vonica@urmc.rochester.edu.
Caroline ZeissCase Western Reserve University, USA. Electronic address: caroline.zeiss@yale.edu.
Kimberly Lezon-GeydaDepartment of Pathology, Yale University, USA. Electronic address: kimberly.lezon-geyda@yale.edu.
Bogdan YatsulaDepartment of Pathology, Yale University, USA. Electronic address: bogdan.yatsula@yale.edu.
David R SellCase Western Reserve University, USA. Electronic address: drs7@cwru.edu.
Vincent M MonnierCase Western Reserve University, USA. Electronic address: vmm3@cwru.edu.
Sharon LinDepartment of Pathology, Yale University, USA. Electronic address: sharon.lin@yale.edu.
Thomas ArditoDepartment of Pathology, Yale University, USA. Electronic address: thomas.ardito@yale.edu.
David EyreUniversity of Washington, Seattle, USA. Electronic address: deyre@u.washington.edu.
David ReynoldsDepartment of Orthopedics, University of Rochester Medical Center, USA. Electronic address: david_reynolds@urmc.rochester.edu.
Zhenqiang YaoDepartment of Pathology and Laboratory Medicine, University of Rochester Medical Center, USA. Electronic address: zhenqiang_yao@urmc.rochester.edu.
Hani A AwadDepartment of Orthopedics, University of Rochester Medical Center, USA; Department of Biomedical Engineering, University of Rochester Medical Center, USA. Electronic address: hani_awad@urmc.rochester.edu.
Hongbo YuDepartment of Pathology and Laboratory Medicine, University of Rochester Medical Center, USA. Electronic address: yhb3508@163.com.
Michael WilsonDepartment of Pathology and Laboratory Medicine, University of Rochester Medical Center, USA. Electronic address: michael_wilson@urmc.rochester.edu.
Sylvie HonnonsDepartment of Pathology and Laboratory Medicine, University of Rochester Medical Center, USA. Electronic address: sylvie.honnons@gmail.com.
Brendan F BoyceDepartment of Pathology and Laboratory Medicine, University of Rochester Medical Center, USA. Electronic address: brendan_boyce@urmc.rochester.edu.
Lianping XingDepartment of Pathology and Laboratory Medicine, University of Rochester Medical Center, USA. Electronic address: lianping_xing@urmc.rochester.edu.
Yi ZhangDepartment of Pathology and Laboratory Medicine, University of Rochester Medical Center, USA. Electronic address: yi_zhang@urmc.rochester.edu.
Archibald S PerkinsDepartment of Pathology and Laboratory Medicine, University of Rochester Medical Center, USA. Electronic address: archibald_perkins@urmc.rochester.edu.
University of Rochester Medical Center · USYale University · USCase Western Reserve University · USSeattle University · US

Funding

YALE CORE CENTER FOR MUSCULOSKELETAL DISORDERSP30AR046032 · NIAMS · YALE UNIVERSITY · PI HOROWITZ, MARK C · 1999 to 2013
$9.0M
Studies on the fate of the OsteoclastR01AR043510 · NIAMS · UNIVERSITY OF TEXAS HLTH SCI CTR SAN ANT · PI BOYCE, BRENDAN F · 1995 to 2025
$8.4M
Engineering scarless repair of flexor tendon injuriesR01AR056696 · NIAMS · UNIVERSITY OF ROCHESTER · PI AWAD, HANI A, BENOIT, DANIELLE S. · 2009 to 2019
$3.6M
The University of Rochester Core Center for Musculoskeletal Biology and MedicineP30AR061307 · NIAMS · UNIVERSITY OF ROCHESTER · PI SCHWARZ, EDWARD M. · 2011 to 2015
$3.0M
Role of TNFalpha in Osteoclast-Mediated Bone LossR01AR048697 · NIAMS · UNIVERSITY OF ROCHESTER · PI XING, LIANPING · 2002 to 2012
$2.6M
Leukemic transformation by the AML1/MDS1/EVI1 ProteinR01CA112188 · NCI · YALE UNIVERSITY · PI PERKINS, ARCHIBALD S. · 2005 to 2009
$1.4M
NCI NIH HHS R01 CA112188NIAMS NIH HHS AR46032NIAMS NIH HHS P30 AR046032NIAMS NIH HHS P30 AR061307NIAMS NIH HHS P30AR061307NIAMS NIH HHS R01 AR043510NIAMS NIH HHS R01 AR048697NIAMS NIH HHS R01 AR056696
6 · The paper itself

Abstract

Recent studies have indicated a role for a MECOM allele in susceptibility to osteoporotic fractures in humans. We have generated a mutation in Mecom in mouse (termed ME(m1)) via lacZ knock-in into the upstream transcription start site for the gene, resulting in disruption of Mds1 and Mds1-Evi1 transcripts, but not of Evi1 transcripts. We demonstrate that ME(m1/m1) mice have severe kyphoscoliosis that is reminiscent of human congenital or primary kyphoscoliosis. ME(m1/m1) mice appear normal at birth, but by 2weeks, they exhibit a slight lumbar lordosis and narrowed intervertebral space. This progresses to severe lordosis with disc collapse and synostosis, together with kyphoscoliosis. Bone formation and strength testing show that ME(m1/m1) mice have normal bone formation and composition but are osteopenic. While endochondral bone development is normal, it is markedly dysplastic in its organization. Electron micrographs of the 1week postnatal intervertebral discs reveals marked disarray of collagen fibers, consistent with an inherent weakness in the non-osseous connective tissue associated with the spine. These findings indicate that lack of ME leads to a complex defect in both osseous and non-osseous musculoskeletal tissues, including a marked vertebral osteopenia, degeneration of the IVD, and disarray of connective tissues, which is likely due to an inherent inability to establish and/or maintain components of these tissues.

Indexed as

Gene DeletionAnimalsBiomechanical PhenomenaBone Diseases, MetabolicCollagenDNA-Binding ProteinsFemaleGene TargetingGenetic LociHedgehog ProteinsHumansIntervertebral DiscKyphosisLordosisLumbar VertebraeMaleCollagenDNA-Binding ProteinsHedgehog Proteinsihh protein, mouseMDS1 and EVI1 Complex Locus ProteinMecom protein, mouseReceptor, Parathyroid Hormone, Type 1Transcription FactorsCongenital kyphoscoliosisMDS1-EVI1Osteoporosis

Identifiers

PMID24316420
PMCPMC4440591
OpenAlexW2075859473

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.