Evidence map›Paper›PMID 24316333›Full record

ArticleJournal of virological methods2014

Use of bacteriophage particles displaying influenza virus hemagglutinin for the detection of hemagglutination-inhibition antibodies.

William Domm, Matthew Brewer, Steven F Baker, Changyong Feng, Luis Martínez-Sobrido, John Treanor, Stephen Dewhurst

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virological methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

  • Erratum issued
5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

William DommDepartment of Microbiology and Immunology, University of Rochester Medical Center, Rochester 14642, NY, USA.
Matthew BrewerDepartment of Microbiology and Immunology, University of Rochester Medical Center, Rochester 14642, NY, USA.
Steven F BakerDepartment of Microbiology and Immunology, University of Rochester Medical Center, Rochester 14642, NY, USA.
Changyong FengDepartment of Biostatistics and Computational Biology, University of Rochester Medical Center, Rochester 14642, NY, USA.
Luis Martínez-SobridoDepartment of Microbiology and Immunology, University of Rochester Medical Center, Rochester 14642, NY, USA.
John TreanorDepartment of Medicine (Infectious Diseases Division), University of Rochester Medical Center, Rochester 14642, NY, USA.
Stephen DewhurstDepartment of Microbiology and Immunology, University of Rochester Medical Center, Rochester 14642, NY, USA. Electronic address: stephen_dewhurst@urmc.rochester.edu.
University of Rochester Medical Center · US

Funding

MULTIDISCIPLINARY TRAINING IN PULMONARY RESEARCHT32HL066988 · NHLBI · UNIVERSITY OF ROCHESTER · PI GEORAS, STEVE N, O'REILLY, MICHAEL A · 2001 to 2021
$8.1M
The T32 Predoctoral Training Program in Immunology (PTPI)T32AI007285 · NIAID · UNIVERSITY OF ROCHESTER · PI Scott Andrew Gerber, JACQUES Robert · 1986 to 2026
$7.6M
Training in HIV Replication and PathogenesisT32AI049815 · NIAID · UNIVERSITY OF ROCHESTER · PI WEDEKIND, JOSEPH E · 2001 to 2020
$3.5M
NHLBI NIH HHS T32 HL066988NIAID NIH HHS HHSN266200700008CNIAID NIH HHS T32 AI007285NIAID NIH HHS T32 AI049815
6 · The paper itself

Abstract

Bacteriophage lambda capsids provide a flexible molecular scaffold that can be engineered to display a wide range of exogenous proteins, including full-length viral glycoproteins produced in eukaryotic cells. One application for such particles lies in the detection of virus-specific antibodies, since they may obviate the need to work with infectious stocks of highly pathogenic or emerging viruses that can pose significant biosafety and biocontainment challenges. Bacteriophage lambda capsids were produced that displayed an insect-cell derived, recombinant H5 influenza virus hemagglutinin (HA) on their surface. The particles agglutinated red blood cells efficiently, in a manner that could be blocked using H5 HA-specific monoclonal antibodies. The particles were then used to develop a modified hemagglutinination-inhibition (HAI) assay, which successfully identified human sera with H5 HA-specific HAI activity. These results demonstrate the utility of HA-displaying bacteriophage capsids for the detection of influenza virus-specific HAI antibodies.

Indexed as

Antibodies, ViralBacteriophage lambdaHemagglutination Inhibition TestsHemagglutinin Glycoproteins, Influenza VirusHumansInfluenza, HumanRecombinant ProteinsAntibodies, Viralhemagglutinin, avian influenza A virusHemagglutinin Glycoproteins, Influenza VirusRecombinant ProteinsBacteriophageH5 HAHemagglutination inhibitionHemagglutininInfluenza virusLambda

Identifiers

PMID24316333
PMCPMC4037851
OpenAlexW2022775275

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.