ArticleBiophysical journal2013
Short-chain phosphoinositide partitioning into plasma membrane models.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 27 citations in OpenAlex.
- PtdIns(3,5)PNature · 2026Article
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- Phosphatidylinositol 3,5-bisphosphate regulates CaTraffic (Copenhagen, Denmark) · 2020Article
- PIRT the TRP Channel Regulating Protein Binds Calmodulin and Cholesterol-Like Ligands.Biomolecules · 2020Article
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- Interaction of the late endo-lysosomal lipid PI(3,5)P2 with the Vph1 isoform of yeast V-ATPase increases its activity and cellular stress tolerance.The Journal of biological chemistry · 2019Article
- Phosphatidylinositol 3,5-bisphosphate regulates the transition between trans-SNARE complex formation and vacuole membrane fusion.Molecular biology of the cell · 2019Article
- Reduced axonal surface expression and phosphoinositide sensitivity in KNeurobiology of disease · 2018Article
- Regulation of thermoTRPs by lipids.Temperature (Austin, Tex.) · 2017Review
- cDICE method produces giant lipid vesicles under physiological conditions of charged lipids and ionic solutions.Soft matter · 2016Article
- A molecular determinant of phosphoinositide affinity in mammalian TRPV channels.Scientific reports · 2016Article
- Measuring distances between TRPV1 and the plasma membrane using a noncanonical amino acid and transition metal ion FRET.The Journal of general physiology · 2016Article
- Phosphoinositide regulation of TRPV1 revisited.Pflugers Archiv : European journal of physiology · 2015Review
- Transient receptor potential melastatin 3 is a phosphoinositide-dependent ion channel.The Journal of general physiology · 2015Article
- Regulation of the transient receptor potential channel TRPM3 by phosphoinositides.The Journal of general physiology · 2015Article
- Mechanism for phosphoinositide selectivity and activation of TRPV1 ion channels.The Journal of general physiology · 2015Article
- Activity and Ca²⁺ regulate the mobility of TRPV1 channels in the plasma membrane of sensory neurons.eLife · 2015Article
- Regulation of TRPV1 ion channel by phosphoinositide (4,5)-bisphosphate: the role of membrane asymmetry.The Journal of biological chemistry · 2014Article
- How membrane partitioning modulates receptor activation: parallel versus serial effects of hydrophobic ligands.Biophysical journal · 2013Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
Phosphoinositides are vital for many cellular signaling processes, and therefore a number of approaches to manipulating phosphoinositide levels in cells or excised patches of cell membranes have been developed. Among the most common is the use of "short-chain" phosphoinositides, usually dioctanoyl phosphoinositol phosphates. We use isothermal titration calorimetry to determine partitioning of the most abundant phosphoinositol phosphates, PI(4)P and PI(4,5)P2 into models of the intracellular and extracellular facing leaflets of neuronal plasma membranes. We show that phosphoinositide mole fractions in the lipid membrane reach physiological levels at equilibrium with reasonable solution concentrations. Finally we explore the consequences of our results for cellular electrophysiology. In particular, we find that TRPV1 is more selective for PI(4,5)P2 than PI(4)P and activated by extremely low membrane mole fractions of PIPs. We conclude by discussing how the logic of our work extends to other experiments with short-chain phosphoinositides. For delayed rectifier K(+) channels, consideration of the membrane mole fraction of PI(4,5)P2 lipids with different acyl chain lengths suggests a different mechanism for PI(4,5)P2 regulation than previously proposed. Inward rectifier K(+) channels apparent lack of selectivity for certain short-chain PIPs may require reinterpretation in view of the PIPs different membrane partitioning.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.