ArticleACS synthetic biology2014
Genetically encoded sender-receiver system in 3D mammalian cell culture.
Article in ACS synthetic biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- A computational modeling approach for predicting multicell spheroid patterns based on signaling-induced differential adhesion.PLoS computational biology · 2022Article
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- Engineering pattern formation and morphogenesis.Biochemical Society transactions · 2020Review
- From Microbial Communities to Distributed Computing Systems.Frontiers in bioengineering and biotechnology · 2020Review
- Massively parallel RNA device engineering in mammalian cells with RNA-Seq.Nature communications · 2019Article
- Programming Morphogenesis through Systems and Synthetic Biology.Trends in biotechnology · 2018Review
- Mammalian synthetic biology for studying the cell.The Journal of cell biology · 2017Review
- Identifying ultrasensitive HGF dose-response functions in a 3D mammalian system for synthetic morphogenesis.Scientific reports · 2016Article
- Engineering cell-based therapies to interface robustly with host physiology.Advanced drug delivery reviews · 2016Review
- Synthetic biology meets tissue engineering.Biochemical Society transactions · 2016Review
- Article
- 2- and 3-dimensional synthetic large-scale de novo patterning by mammalian cells through phase separation.Scientific reports · 2016Article
- A synthetic mammalian network to compute population borders based on engineered reciprocal cell-cell communication.BMC systems biology · 2015Article
- Cooperativity to increase Turing pattern space for synthetic biology.ACS synthetic biology · 2015Article
- Sender-receiver systems and applying information theory for quantitative synthetic biology.Current opinion in biotechnology · 2015Review
- Artificial cell-cell communication as an emerging tool in synthetic biology applications.Journal of biological engineering · 2015Article
- Synthetic biology and therapeutic strategies for the degenerating brain: Synthetic biology approaches can transform classical cell and gene therapies, to provide new cures for neurodegenerative diseases.BioEssays : news and reviews in molecular, cellular and developmental biology · 2014Review
- A library of mammalian effector modules for synthetic morphology.Journal of biological engineering · 2014Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Engineering spatial patterning in mammalian cells, employing entirely genetically encoded components, requires solving several problems. These include how to code secreted activator or inhibitor molecules and how to send concentration-dependent signals to neighboring cells, to control gene expression. The Madin-Darby Canine Kidney (MDCK) cell line is a potential engineering scaffold as it forms hollow spheres (cysts) in 3D culture and tubulates in response to extracellular hepatocyte growth factor (HGF). We first aimed to graft a synthetic patterning system onto single developing MDCK cysts. We therefore developed a new localized transfection method to engineer distinct sender and receiver regions. A stable reporter line enabled reversible EGFP activation by HGF and modulation by a secreted repressor (a truncated HGF variant, NK4). By expanding the scale to wide fields of cysts, we generated morphogen diffusion gradients, controlling reporter gene expression. Together, these components provide a toolkit for engineering cell-cell communication networks in 3D cell culture.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.