ArticleAdvanced pharmaceutical bulletin2013
A unique report: development of super anti-human IgG monoclone with optical density over than 3.
Article in Advanced pharmaceutical bulletin, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- PCSK9: A Key Target for the Treatment of Cardiovascular Disease (CVD).Advanced pharmaceutical bulletin · 2020Review
- A methodological approach for production and purification of polyclonal antibody against dog IgG.Veterinary research forum : an international quarterly journal · 2018Article
- Production and purification of polyclonal antibody against F(ab')2 fragment of human immunoglobulin G.Veterinary research forum : an international quarterly journal · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
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Abstract
purposeMonoclonal antibodies and related conjugates are key reagents used in biomedical researches as well as, in treatment, purification and diagnosis of infectious and non- infectious diseases.
methodsBalb/c mice were immunized with purified human IgG. Spleen cells of the most immune mouse were fused with SP2/0 in the presence of Poly Ethylene Glycol (PEG). Supernatant of hybridoma cells was screened for detection of antibody by ELISA. Then, the sample was assessed for cross-reactivity with IgM & IgA by ELISA and confirmed by immunoblotting. The subclasses of the selected mAbs were determined. The best clone was injected intraperitoneally to some pristane-injected mice. Anti-IgG mAb was purified from the animals' ascitic fluid by Ion exchange chromatography and then, mAb was conjugated with HRP.
resultsIn the present study, over than 50 clones were obtained that 1 clone had optical density over than 3. We named this clone as supermonoclone which was selected for limiting dilution. The result of the immunoblotting, showed sharp band in IgG position and did not show any band in IgM&IgA position.
conclusionBased on the findings of this study, the conjugated monoclonal antibody could have application in diagnosis of infectious diseases like Toxoplasmosis, Rubella and IgG class of other infectious and non- infectious diseases.
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