ArticleAdvanced pharmaceutical bulletin2013
Large Scale Generation and Characterization of Anti-Human CD34 Monoclonal Antibody in Ascetic Fluid of Balb/c Mice.
Article in Advanced pharmaceutical bulletin, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Expression and Purification of a PEDV-Neutralizing Antibody and Its Functional Verification.Viruses · 2021Article
- PCSK9: A Key Target for the Treatment of Cardiovascular Disease (CVD).Advanced pharmaceutical bulletin · 2020Review
- Diagnosis of hepatocellular carcinoma using a novel anti-glycocholic acid monoclonal antibody-based method.Oncology letters · 2019Article
- Purification of a Novel Anti-VEGFR2 Single Chain Antibody Fragment and Evaluation of Binding Affinity by Surface Plasmon Resonance.Advanced pharmaceutical bulletin · 2019Article
- Antibody-drug conjugates: Promising and efficient tools for targeted cancer therapy.Journal of cellular physiology · 2018Review
- A methodological approach for production and purification of polyclonal antibody against dog IgG.Veterinary research forum : an international quarterly journal · 2018Article
- Production and purification of polyclonal antibody against F(ab')2 fragment of human immunoglobulin G.Veterinary research forum : an international quarterly journal · 2017Article
- Overview of Albumin and Its Purification Methods.Advanced pharmaceutical bulletin · 2016Review
- Physicochemical Evaluation of Lyophilized Formulation ofIranian journal of pharmaceutical research : IJPR · 2016Article
- Production and Purification of a Polyclonal Antibody Against Purified Mouse IgG2b in Rabbits Towards Designing Mouse Monoclonal Isotyping Kits.Advanced pharmaceutical bulletin · 2015Article
- Large scale generation and characterization of anti-human IgA monoclonal antibody in ascitic fluid of BALB/c mice.Advanced pharmaceutical bulletin · 2015Article
- Generation and Characterization of Anti-CD34 Monoclonal Antibodies that React with Hematopoietic Stem Cells.Cell journal · 2014Article
- A unique report: development of super anti-human IgG monoclone with optical density over than 3.Advanced pharmaceutical bulletin · 2013Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeMonoclonal antibodies or specific antibodies are now an essential tool of biomedical research and are of great commercial and medical value. The purpose of this study was to produce large scale of monoclonal antibody against CD34 in order to diagnostic application in leukemia and purification of human hematopoietic stem/progenitor cells.
methodsFor large scale production of monoclonal antibody, hybridoma cells that produce monoclonal antibody against human CD34 were injected into the peritoneum of the Balb/c mice which have previously been primed with 0.5 ml Pristane. 5 ml ascitic fluid was harvested from each mouse in two times. Evaluation of mAb titration was assessed by ELISA method. The ascitic fluid was examined for class and subclasses by ELISA mouse mAb isotyping Kit. mAb was purified from ascitic fluid by affinity chromatography on Protein A-Sepharose. Purity of monoclonal antibody was monitored by SDS -PAGE and the purified monoclonal antibody was conjugated with FITC.
resultsMonoclonal antibodies with high specificity and sensitivity against human CD34 by hybridoma technology were prepared. The subclass of antibody was IgG1 and its light chain was kappa.
conclusionThe conjugated monoclonal antibody could be a useful tool for isolation, purification and characterization of human hematopoietic stem cells.
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